• Immunofluorescence analysis of U-2 OS cells using JNK1/JNK3 Rabbit monoclonal antibody (STJ11102232) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using JNK1/JNK3 Rabbit monoclonal antibody (STJ11102232) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using JNK1/JNK3 Rabbit monoclonal antibody (STJ11102232) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of JNK1/JNK3 in paraffin-embedded human colon using JNK1/JNK3 Rabbit monoclonal antibody (STJ11102232) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using JNK1/JNK3 Rabbit monoclonal antibody (STJ11102232) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.

Anti-MAPK8 antibody (1-383) [S2MR] (STJ11102232)

SKU:
STJ11102232

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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAPK8
MAPK10
Gene ID: 5599
5602
Uniprot ID: MK08_HUMAN
MK10_HUMAN
Immunogen Region: 1-383
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-383 of human JNK1 (P45983).This antibody does not cross react with JNK2 protein.
Immunogen Sequence: MSRSKRDNNFYSVEIGDSTF TVLKRYQNLKPIGSGAQGIV CAAYDAILERNVAIKKLSRP FQNQTHAKRAYRELVLMKCV NHKNIIGLLNVFTPQKSLEE FQDVYIVMELMDANLCQVIQ MELDHERMSYLLYQMLCGIK HLHSAGIIHRDLKPSNIVVK SDCTLKILDFGLARTAGTSF MMTPYVVTRYYRAPEVILGM GYKENVDLWSVGCIMGEMVC HKILFPGRDYIDQWNKVIE
Tissue Specificity P45983 - NA
P53779 - Specific to a subset of neurons in the nervous system. Present in the hippocampus and areas, cerebellum, striatum, brain stem, and weakly in the spinal cord. Very weak expression in testis and kidney.
Alternative Antibody Names Anti-Mitogen-Activated Protein Kinase 8 antibody
Anti-Map Kinase 8 antibody
Anti-Mapk 8 antibody
Anti-Jnk-46 antibody
Anti-Stress-Activated Protein Kinase 1c antibody
Anti-Sapk1c antibody
Anti-Stress-Activated Protein Kinase Jnk1 antibody
Anti-C-Jun N-Terminal Kinase 1 antibody
Anti-MAPK8 antibody
Anti-JNK1 antibody
Anti-PRKM8 antibody
Anti-SAPK1 antibody
Anti-SAPK1C antibody
Anti-Mitogen-Activated Protein Kinase 10 antibody
Anti-Map Kinase 10 antibody
Anti-Mapk 10 antibody
Anti-Map Kinase P49 3f12 antibody
Anti-Stress-Activated Protein Kinase 1b antibody
Anti-Sapk1b antibody
Anti-Stress-Activated Protein Kinase Jnk3 antibody
Anti-C-Jun N-Terminal Kinase 3 antibody
Anti-MAPK10 antibody
Anti-JNK3 antibody
Anti-JNK3A antibody
Anti-PRKM10 antibody
Anti-SAPK1B antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance