| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:2000-1:5000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MAPK12 |
| Gene ID: | 6300 |
| Uniprot ID: | MK12_HUMAN |
| Immunogen Region: | 248-367 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 248-367 of human MAPK12 (NP_002960.2). |
| Immunogen Sequence: | EFVQRLQSDEAKNYMKGLPE LEKKDFASILTNASPLAVNL LEKMLVLDAEQRVTAGEALA HPYFESLHDTEDEPQVQKYD DSFDDVDRTLDEWKRVTYKE VLSFKPPRQLGARVSKETPL |
| Tissue Specificity | Highly expressed in skeletal muscle and heart. |
| Post Translational Modifications | Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K3/MKK3 and MAP2K6/MKK6, which activates the enzyme. Ubiquitinated. Ubiquitination leads to degradation by the proteasome pathway. |
| Function | Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK12 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as pro-inflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets. Plays a role in myoblast differentiation and also in the down-regulation of cyclin D1 in response to hypoxia in adrenal cells suggesting MAPK12 may inhibit cell proliferation while promoting differentiation. Phosphorylates DLG1. Following osmotic shock, MAPK12 in the cell nucleus increases its association with nuclear DLG1, thereby causing dissociation of DLG1-SFPQ complexes. This function is independent of its catalytic activity and could affect mRNA processing and/or gene transcription to aid cell adaptation to osmolarity changes in the environment. Regulates UV-induced checkpoint signaling and repair of UV-induced DNA damage and G2 arrest after gamma-radiation exposure. MAPK12 is involved in the regulation of SLC2A1 expression and basal glucose uptake in L6 myotubes.and negatively regulates SLC2A4 expression and contraction-mediated glucose uptake in adult skeletal muscle. C-Jun (JUN) phosphorylation is stimulated by MAPK14 and inhibited by MAPK12, leading to a distinct AP-1 regulation. MAPK12 is required for the normal kinetochore localization of PLK1, prevents chromosomal instability and supports mitotic cell viability. MAPK12-signaling is also positively regulating the expansion of transient amplifying myogenic precursor cells during muscle growth and regeneration. |
| Protein Name | Mitogen-Activated Protein Kinase 12 Map Kinase 12 Mapk 12 Extracellular Signal-Regulated Kinase 6 Erk-6 Mitogen-Activated Protein Kinase P38 Gamma Map Kinase P38 Gamma Stress-Activated Protein Kinase 3 |
| Database Links | Reactome: R-HSA-168638 Reactome: R-HSA-171007 Reactome: R-HSA-2151209 Reactome: R-HSA-4420097 Reactome: R-HSA-525793 Reactome: R-HSA-5675221 |
| Cellular Localisation | Cytoplasm Nucleus Mitochondrion Mitochondrial When Associated With Sh3bp5 In Skeletal Muscle Colocalizes With Snta1 At The Neuromuscular Junction And Throughout The Sarcolemma |
| Alternative Antibody Names | Anti-Mitogen-Activated Protein Kinase 12 antibody Anti-Map Kinase 12 antibody Anti-Mapk 12 antibody Anti-Extracellular Signal-Regulated Kinase 6 antibody Anti-Erk-6 antibody Anti-Mitogen-Activated Protein Kinase P38 Gamma antibody Anti-Map Kinase P38 Gamma antibody Anti-Stress-Activated Protein Kinase 3 antibody Anti-MAPK12 antibody Anti-ERK6 antibody Anti-SAPK3 antibody |
Information sourced from Uniprot.org