Host: | Rabbit |
Applications: | WB/IHC/IF/ELISA |
Reactivity: | Human/Mouse/Rat |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Rabbit polyclonal antibody anti-Mitogen-activated protein kinase 1 (136-185 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
Clonality: | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Concentration: | 1 mg/mL |
Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 IF 1:200-1:1000 ELISA 1:5000 |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: | MAPK1 |
Gene ID: | 5594 |
Uniprot ID: | MK01_HUMAN |
Immunogen Region: | 136-185 aa |
Specificity: | ERK 2 Polyclonal Antibody detects endogenous levels of ERK 2 protein. |
Immunogen: | The antiserum was produced against synthesized peptide derived from the human p42 MAPK at the amino acid range 136-185 |
Function | Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC).as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1 and FXR1) and a variety of other signaling-related molecules (like ARHGEF2, DCC, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Mediates phosphorylation of TPR in response to EGF stimulation. May play a role in the spindle assembly checkpoint. Phosphorylates PML and promotes its interaction with PIN1, leading to PML degradation. Phosphorylates CDK2AP2. Acts as a transcriptional repressor. Binds to a GCAAAGC consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity. |
Protein Name | Mitogen-Activated Protein Kinase 1 Map Kinase 1 Mapk 1 Ert1 Extracellular Signal-Regulated Kinase 2 Erk-2 Map Kinase Isoform P42 P42-Mapk Mitogen-Activated Protein Kinase 2 Map Kinase 2 Mapk 2 |
Database Links | Reactome: R-HSA-111995 Reactome: R-HSA-112409 Reactome: R-HSA-112411 Reactome: R-HSA-1181150 Reactome: R-HSA-1295596 Reactome: R-HSA-1502540 Reactome: R-HSA-162658 Reactome: R-HSA-170968 Reactome: R-HSA-198753 Reactome: R-HSA-202670 Reactome: R-HSA-2029482 Reactome: R-HSA-2173795 Reactome: R-HSA-2173796 Reactome: R-HSA-2559580 Reactome: R-HSA-2559582 Reactome: R-HSA-2559585 Reactome: R-HSA-2871796 Reactome: R-HSA-3371453 Reactome: R-HSA-375165 Reactome: R-HSA-437239 Reactome: R-HSA-444257 Reactome: R-HSA-445144 Reactome: R-HSA-450341 Reactome: R-HSA-456926 Reactome: R-HSA-5654726 Reactome: R-HSA-5654727 Reactome: R-HSA-5654732 Reactome: R-HSA-5654733 Reactome: R-HSA-5663213 Reactome: R-HSA-5668599 Reactome: R-HSA-5673001 Reactome: R-HSA-5674135 Reactome: R-HSA-5674499 Reactome: R-HSA-5675221 Reactome: R-HSA-6798695 Reactome: R-HSA-6802946 Reactome: R-HSA-6802948 Reactome: R-HSA-6802952 Reactome: R-HSA-6802955 Reactome: R-HSA-6811558 Reactome: R-HSA-74749 Reactome: R-HSA-877300 Reactome: R-HSA-879415 Reactome: R-HSA-881907 Reactome: R-HSA-8939211 Reactome: R-HSA-8940973 Reactome: R-HSA-8943724 Reactome: R-HSA-9627069 Reactome: R-HSA-9634635 Reactome: R-HSA-9634638 Reactome: R-HSA-9635465 Reactome: R-HSA-9649948 Reactome: R-HSA-9652169 Reactome: R-HSA-9652817 Reactome: R-HSA-9656223 Reactome: R-HSA-9664422 Reactome: R-HSA-9725371 Reactome: R-HSA-9732724 Reactome: R-HSA-9768919 Reactome: R-HSA-982772 Reactome: R-HSA-9842640 Reactome: R-HSA-9856649 |
Cellular Localisation | Cytoplasm Cytoskeleton Spindle Nucleus Microtubule Organizing Center Centrosome Membrane Caveola Cell Junction Focal Adhesion Associated With The Spindle During Prometaphase And Metaphase Pea15-Binding And Phosphorylated Dapk1 Promote Its Cytoplasmic Retention Phosphorylation At Ser- 246 And Ser-248 As Well As Autophosphorylation At Thr-190 Promote Nuclear Localization |
Alternative Antibody Names | Anti-Mitogen-Activated Protein Kinase 1 antibody Anti-Map Kinase 1 antibody Anti-Mapk 1 antibody Anti-Ert1 antibody Anti-Extracellular Signal-Regulated Kinase 2 antibody Anti-Erk-2 antibody Anti-Map Kinase Isoform P42 antibody Anti-P42-Mapk antibody Anti-Mitogen-Activated Protein Kinase 2 antibody Anti-Map Kinase 2 antibody Anti-Mapk 2 antibody Anti-MAPK1 antibody Anti-ERK2 antibody Anti-PRKM1 antibody Anti-PRKM2 antibody |
Information sourced from Uniprot.org