| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Mitogen-activated protein kinase kinase kinase kinase 1 (371-420 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 ELISA 1:10000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MAP4K1 |
| Gene ID: | 11184 |
| Uniprot ID: | M4K1_HUMAN |
| Immunogen Region: | 371-420 aa |
| Specificity: | HPK1 Polyclonal Antibody detects endogenous levels of HPK1 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human MEKKK 1 at the amino acid range 371-420 |
| Post Translational Modifications | Autophosphorylates: phosphorylation promotes ubiquitination by the Cul7-RING(FBXW8) ubiquitin-protein ligase complex, leading to its degradation by the proteasome. Tyrosine-phosphorylated after activation of hemopoietic cells. Ubiquitinated by the Cul7-RING(FBXW8) ubiquitin-protein ligase complex following autophosphorylation, leading to its degradation by the proteasome. |
| Function | Serine/threonine-protein kinase, which plays a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. Activator of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. MAP4Ks act in parallel to and are partially redundant with STK3/MST2 and STK4/MST2 in the phosphorylation and activation of LATS1/2, and establish MAP4Ks as components of the expanded Hippo pathway. May play a role in hematopoietic lineage decisions and growth regulation. Together with CLNK, it enhances CD3-triggered activation of T-cells and subsequent IL2 production. |
| Protein Name | Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 Hematopoietic Progenitor Kinase Mapk/Erk Kinase Kinase Kinase 1 Mek Kinase Kinase 1 Mekkk 1 |
| Alternative Antibody Names | Anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 antibody Anti-Hematopoietic Progenitor Kinase antibody Anti-Mapk/Erk Kinase Kinase Kinase 1 antibody Anti-Mek Kinase Kinase 1 antibody Anti-Mekkk 1 antibody Anti-MAP4K1 antibody Anti-HPK1 antibody |
Information sourced from Uniprot.org
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Anti-HPK1 antibody (STJ93590)
Application Used: Western Blot Tissue: LY3, JEKO, Granta Dilution: 1:1000 Secondary Antibody: Amersham ECL Rabbit IgG, HRP-linked whole Ab (from donkey) Secondary Antibody Dilution: 1:2500 Gel Electrophoresis: 6% polyacrylamide gel, constant voltage 20 V, overnight. Protein Transfer: 0.45 nm nitrocellulose membrane, 1 piece of gel (wet transfer), constant voltage 20 V overnight Blocking: 1X TBST with 5% Bovine serum albumin (BSA) for 2 hr. Primary Probing: Primary antibody was added to the solution of 1X TBST with 5% BSA, 4˚C for overnight. Washing: 3 times 1X TBST wash for 5 minutes. Secondary Probing: Secondary antibody was added to the solution of 1X TBST with 5% BSA with 1:2500 dilution ratio, for 2 hr. Visualisation: ECL visualization for 1 min Findings: Its good to detect total as well as cleaved HPK1 antibody
