![Western blot analysis of lysates from wild type (WT) and MAP3K1 knockout (KO) HeLa cells, using [KO Validated] MAP3K1 Rabbit polyclonal antibody (STJ11100014) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89087/357547/STJ11100014_1__92691.1713121666.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MAP3K1 knockout (KO) HeLa cells, using [KO Validated] MAP3K1 Rabbit polyclonal antibody (STJ11100014) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
![Western blot analysis of lysates from wild type (WT) and MAP3K1 knockout (KO) HeLa cells, using [KO Validated] MAP3K1 Rabbit polyclonal antibody (STJ11100014) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89087/357547/STJ11100014_1__92691.1713121666.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MAP3K1 knockout (KO) HeLa cells, using [KO Validated] MAP3K1 Rabbit polyclonal antibody (STJ11100014) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IHC-P:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MAP3K1 |
| Gene ID: | 4214 |
| Uniprot ID: | M3K1_HUMAN |
| Immunogen Region: | 1400-1512 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 1400-1512 of human MAP3K1 (NP_005912.1). |
| Immunogen Sequence: | TGAGEFQGQLLGTIAFMAPE VLRGQQYGRSCDVWSVGCAI IEMACAKPPWNAEKHSNHLA LIFKIASATTAPSIPSHLSP GLRDVALRCLELQPQDRPPS RELLKHPVFRTTW |
| Post Translational Modifications | Autophosphorylated. |
| Function | Component of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. May phosphorylate the MAPK8/JNK1 kinase. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway. |
| Protein Name | Mitogen-Activated Protein Kinase Kinase Kinase 1 Mapk/Erk Kinase Kinase 1 Mek Kinase 1 Mekk 1 |
| Database Links | Reactome: R-HSA-166058 Reactome: R-HSA-2871796 Reactome: R-HSA-933542 Reactome: R-HSA-975138 Reactome: R-HSA-975871 |
| Alternative Antibody Names | Anti-Mitogen-Activated Protein Kinase Kinase Kinase 1 antibody Anti-Mapk/Erk Kinase Kinase 1 antibody Anti-Mek Kinase 1 antibody Anti-Mekk 1 antibody Anti-MAP3K1 antibody Anti-MAPKKK1 antibody Anti-MEKK antibody Anti-MEKK1 antibody |
Information sourced from Uniprot.org