| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-MEK1/MEK2 (1-360) is suitable for use in Western Blot research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S5MR |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:2000-1:6000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunogen Region: |
1-360 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-360 of human MEK1/MEK2 (Q02750). |
| Immunogen Sequence: |
MPKKKPTPIQLNPAPDGSAV NGTSSAETNLEALQKKLEEL ELDEQQRKRLEAFLTQKQKV GELKDDDFEKISELGAGNGG VVFKVSHKPSGLVMARKLIH LEIKPAIRNQIIRELQVLHE CNSPYIVGFYGAFYSDGEIS ICMEHMDGGSLDQVLKKAGR IPEQILGKVSIAVIKGLTYL REKHKIMHRDVKPSNILVNS RGEIKLCDFGVSGQLIDSMA NSFVGTRSYMSPERLQGTH |
| Background | The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus. |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of various lysates using [KO Validated] MEK2 Rabbit monoclonal antibody (STJ11101615) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/90529/360110/STJ11101615_1__89635.1713124721.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] MEK2 Rabbit monoclonal antibody (STJ11101615) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunofluorescence analysis of U2OS cells using [KO Validated] MEK2 Rabbit polyclonal antibody (STJ116970) at dilution of 1:100. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/95937/378188/STJ116970_1__00761.1713145688.jpg?c=1 "Immunofluorescence analysis of U2OS cells using [KO Validated] MEK2 Rabbit polyclonal antibody (STJ116970) at dilution of 1:100. Blue: DAPI for nuclear staining.")