• Immunohistochemistry analysis of LC3A/ LC3B in paraffin-embedded human mammary cancer using LC3A/ LC3B Rabbit polyclonal antibody (STJ117779) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of LC3A/ LC3B in paraffin-embedded human gastric cancer using LC3A/ LC3B Rabbit polyclonal antibody (STJ117779) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of LC3A/ LC3B in paraffin-embedded human breast cancer using LC3A/ LC3B Rabbit polyclonal antibody (STJ117779) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from HeLa cells using LC3A/ LC3B Rabbit polyclonal antibody (STJ117779) at 1:1000 dilution incubated at room temperature for 1. 5 hours. Hela cells were treated by Chloroquine (50 Mu M) at 37 °C for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 30 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of lysates from NIH/3T3 cells using LC3A/ LC3B Rabbit polyclonal antibody (STJ117779) at 1:1000 dilution incubated at room temperature for 1. 5 hours. NIH/3T3 cells were treated by Chloroquine (50 Mu M) at 37 °C for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 30 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-MAP1LC3A/MAP1LC3B antibody (1-125) (STJ117779)

SKU:
STJ117779

£112.50 - £522.50
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Host: Rabbit
Applications: IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LC3A/LC3B (1-125) is suitable for use in Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 1-125
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human LC3A/LC3B (NP_073729.1).
Immunogen Sequence: MPSEKTFKQRRTFEQRVEDV RLIREQHPTKIPVIIERYKG EKQLPVLDKTKFLVPDHVNM SELIKIIRRRLQLNANQAFF LLVNGHSMVSVSTPISEVYE SEKDEDGFLYMVYASQETFG MKLSV
Background The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The expression of this gene is restricted to small intestine, colon, and rectum, and it is underexpressed in colorectal cancer.

Information sourced from Uniprot.org

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