• Immunohistochemistry analysis of LC3A/ LC3B in paraffin-embedded human mammary cancer using LC3A/ LC3B Rabbit polyclonal antibody (STJ117779) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of LC3A/ LC3B in paraffin-embedded human gastric cancer using LC3A/ LC3B Rabbit polyclonal antibody (STJ117779) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of LC3A/ LC3B in paraffin-embedded human breast cancer using LC3A/ LC3B Rabbit polyclonal antibody (STJ117779) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from HeLa cells using LC3A/ LC3B Rabbit polyclonal antibody (STJ117779) at 1:1000 dilution incubated at room temperature for 1. 5 hours. Hela cells were treated by Chloroquine (50 Mu M) at 37 °C for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 30 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of lysates from NIH/3T3 cells using LC3A/ LC3B Rabbit polyclonal antibody (STJ117779) at 1:1000 dilution incubated at room temperature for 1. 5 hours. NIH/3T3 cells were treated by Chloroquine (50 Mu M) at 37 °C for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 30 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-MAP1LC3A/MAP1LC3B antibody (1-125) (STJ117779)

SKU:
STJ117779

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAP1LC3B
MAP1LC3A
Gene ID: 81631
84557
Uniprot ID: MLP3B_HUMAN
MLP3A_HUMAN
Immunogen Region: 1-125
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human LC3A/LC3B (NP_073729.1).
Immunogen Sequence: MPSEKTFKQRRTFEQRVEDV RLIREQHPTKIPVIIERYKG EKQLPVLDKTKFLVPDHVNM SELIKIIRRRLQLNANQAFF LLVNGHSMVSVSTPISEVYE SEKDEDGFLYMVYASQETFG MKLSV
Tissue Specificity Q9GZQ8 - Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.
Q9H492 - Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
Alternative Antibody Names Anti-Microtubule-Associated Proteins 1a/1b Light Chain 3b antibody
Anti-Autophagy-Related Protein Lc3 B antibody
Anti-Autophagy-Related Ubiquitin-Like Modifier Lc3 B antibody
Anti-Map1 Light Chain 3-Like Protein 2 antibody
Anti-Map1a/Map1b Light Chain 3 B antibody
Anti-Map1a/Map1b Lc3 B antibody
Anti-Microtubule-Associated Protein 1 Light Chain 3 Beta antibody
Anti-MAP1LC3B antibody
Anti-MAP1ALC3 antibody
Anti-Microtubule-Associated Proteins 1a/1b Light Chain 3a antibody
Anti-Autophagy-Related Protein Lc3 A antibody
Anti-Autophagy-Related Ubiquitin-Like Modifier Lc3 A antibody
Anti-Map1 Light Chain 3-Like Protein 1 antibody
Anti-Map1a/Map1b Light Chain 3 A antibody
Anti-Map1a/Map1b Lc3 A antibody
Anti-Microtubule-Associated Protein 1 Light Chain 3 Alpha antibody
Anti-MAP1LC3A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance