Western blot analysis of lysates from mouse liver, using MAN2B1 Rabbit pAb (STJ111978) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 30s.

Anti-MAN2B1 antibody (640-850) (STJ111978)

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STJ111978
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAN2B1
Gene ID: 4125
Uniprot ID: MA2B1_HUMAN
Immunogen Region: 640-850
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 640-850 of human MAN2B1 (NP_000519.2).
Immunogen Sequence: TFFWYNASIGDNESDQASGA YIFRPNQQKPLPVSRWAQIH LVKTPLVQEVHQNFSAWCSQ VVRLYPGQRHLELEWSVGPI PVGDTWGKEVISRFDTPLET KGRFYTDSNGREILERRRDY RPTWKLNQTEPVAGNYYPVN TRIYITDGNMQLTVLTDRSQ GGSSLRDGSLELMVHRRLLK DDGRGVSEPLMENGSGAWVR GRHLVLLDTAQ
Post Translational Modifications First processed into 3 peptides of 70 kDa, 42 kDa (D) and 13/15 kDa (E). The 70 kDa peptide is further processed into three peptides (A, B and C). The A, B and C peptides are disulfide-linked. Heavily glycosylated.
Function Necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover. Cleaves all known types of alpha-mannosidic linkages.
Protein Name Lysosomal Alpha-Mannosidase
Laman
Lysosomal Acid Alpha-Mannosidase
Mannosidase Alpha Class 2b Member 1
Mannosidase Alpha-B Cleaved Into - Lysosomal Alpha-Mannosidase A Peptide - Lysosomal Alpha-Mannosidase B Peptide - Lysosomal Alpha-Mannosidase C Peptide - Lysosomal Alpha-Mannosidase D Peptide - Lysosomal Alpha-Mannosidase E Peptide
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-8853383
Cellular Localisation Lysosome
Alternative Antibody Names Anti-Lysosomal Alpha-Mannosidase antibody
Anti-Laman antibody
Anti-Lysosomal Acid Alpha-Mannosidase antibody
Anti-Mannosidase Alpha Class 2b Member 1 antibody
Anti-Mannosidase Alpha-B Cleaved Into - Lysosomal Alpha-Mannosidase A Peptide - Lysosomal Alpha-Mannosidase B Peptide - Lysosomal Alpha-Mannosidase C Peptide - Lysosomal Alpha-Mannosidase D Peptide - Lysosomal Alpha-Mannosidase E Peptide antibody
Anti-MAN2B1 antibody
Anti-LAMAN antibody
Anti-MANB antibody

Information sourced from Uniprot.org

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