• Western blot analysis of various lysates using MAGEA4 Rabbit polyclonal antibody (STJ113212) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-MAGEA4 antibody (28-317) (STJ113212)

SKU:
STJ113212

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MAGEA4 (28-317) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAGEA4
Gene ID: 4103
Uniprot ID: MAGA4_HUMAN
Immunogen Region: 28-317
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 28-317 of human MAGEA4 (NP_002353.3).
Immunogen Sequence: AQAPTTEEQEAAVSSSSPLV PGTLEEVPAAESAGPPQSPQ GASALPTTISFTCWRQPNEG SSSQEEEGPSTSPDAESLFR EALSNKVDELAHFLLRKYRA KELVTKAEMLERVIKNYKRC FPVIFGKASESLKMIFGIDV KEVDPASNTYTLVTCLGLSY DGLLGNNQIFPKTGLLIIVL GTIAMEGDSASEEEIWEELG VMGVYDGREHTVYGEPRKLL TQDWVQENYLEYRQVPGSN
Tissue Specificity Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta.
Function Regulates cell proliferation through the inhibition of cell cycle arrest at the G1 phase. Also negatively regulates p53-mediated apoptosis.
Protein Name Melanoma-Associated Antigen 4
Cancer/Testis Antigen 1.4
Ct1.4
Mage-4 Antigen
Mage-41 Antigen
Mage-X2 Antigen
Alternative Antibody Names Anti-Melanoma-Associated Antigen 4 antibody
Anti-Cancer/Testis Antigen 1.4 antibody
Anti-Ct1.4 antibody
Anti-Mage-4 Antigen antibody
Anti-Mage-41 Antigen antibody
Anti-Mage-X2 Antigen antibody
Anti-MAGEA4 antibody
Anti-MAGE4 antibody

Information sourced from Uniprot.org

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