| Host: |
Rabbit |
| Applications: |
WB/IP/ELISA |
| Reactivity: |
Human/Rat/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Mitotic spindle assembly checkpoint protein MAD2A (91-140 aa) is suitable for use in Western Blot, Immunoprecipitation and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000IP 1:200-500ELISA 1:5000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MAD2L1 |
| Gene ID: |
4085 |
| Uniprot ID: |
MD2L1_HUMAN |
| Immunogen Region: |
91-140 aa |
| Specificity: |
MAD2 Polyclonal Antibody detects endogenous levels of MAD2 protein. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human MAD2L1 at the amino acid range 91-140 |
| Function | Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. In the closed conformation (C-MAD2) forms a heterotetrameric complex with MAD1L1 at unattached kinetochores during prometaphase, the complex recruits open conformation molecules of MAD2L1 (O-MAD2) and then promotes the conversion of O-MAD2 to C-MAD2. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. |
| Protein Name | Mitotic Spindle Assembly Checkpoint Protein Mad2aHsmad2Mitotic Arrest Deficient 2-Like Protein 1Mad2-Like Protein 1 |
| Database Links | Reactome: R-HSA-141405Reactome: R-HSA-141430Reactome: R-HSA-141444Reactome: R-HSA-174184Reactome: R-HSA-176409Reactome: R-HSA-179409Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-5663220Reactome: R-HSA-68877Reactome: R-HSA-9648025 |
| Cellular Localisation | NucleusChromosomeCentromereKinetochoreCytoplasmCytoskeletonSpindle PoleRecruited By Mad1l1 To Unattached Kinetochores (Probable)Recruited To The Nuclear Pore Complex By Tpr During InterphaseRecruited To Kinetochores In Late Prometaphase After Bub1CenpfBub1b And CenpeKinetochore Association Requires The Presence Of Nek2Kinetochore Association Is Repressed By UbdSequestered To The Cytoplasm Upon Interaction With Isoform 3 Of Mad1l1 |
| Alternative Antibody Names | Anti-Mitotic Spindle Assembly Checkpoint Protein Mad2a antibodyAnti-Hsmad2 antibodyAnti-Mitotic Arrest Deficient 2-Like Protein 1 antibodyAnti-Mad2-Like Protein 1 antibodyAnti-MAD2L1 antibodyAnti-MAD2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
