Western blot analysis of various lysates using LYPLA2 Rabbit polyclonal antibody (STJ118237) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-LYPLA2 antibody (147-231) (STJ118237)

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STJ118237
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LYPLA2
Gene ID: 11313
Uniprot ID: LYPA2_HUMAN
Immunogen Region: 147-231
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 147-231 of human LYPLA2 (NP_009191.1).
Immunogen Sequence: CWLPLHRAFPQAANGSAKDL AILQCHGELDPMVPVRFGAL TAEKLRSVVTPARVQFKTYP GVMHSSCPQEMAAVKEFLEK LLPPV
Tissue Specificity Expressed in various breast cancer cell lines.
Function Acts as an acyl-protein thioesterase hydrolyzing fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins, GSDMD, GAP43, ZDHHC6 or HRAS. Deacylates GAP43. Mediates depalmitoylation of ZDHHC6. Has lysophospholipase activity. Hydrolyzes prostaglandin glycerol esters (PG-Gs) in the following order prostaglandin D2-glycerol ester (PGD2-G) > prostaglandin E2 glycerol ester (PGE2-G) > prostaglandin F2-alpha-glycerol ester (PGF2-alpha-G). Hydrolyzes 1-arachidonoylglycerol but not 2-arachidonoylglycerol or arachidonoylethanolamide.
Protein Name Acyl-Protein Thioesterase 2
Apt-2
Lysophospholipase Ii
Lpl-Ii
Lysopla Ii
Palmitoyl-Protein Hydrolase
Database Links Reactome: R-HSA-373760
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Acyl-Protein Thioesterase 2 antibody
Anti-Apt-2 antibody
Anti-Lysophospholipase Ii antibody
Anti-Lpl-Ii antibody
Anti-Lysopla Ii antibody
Anti-Palmitoyl-Protein Hydrolase antibody
Anti-LYPLA2 antibody
Anti-APT2 antibody

Information sourced from Uniprot.org

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