Western blot analysis of extracts of various cell lines, using Ly-6A/E (Sca-1) antibody (STJ11103169) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Ly-6A/E antibody (1-100) [S9MR] (STJ11103169)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11103169
๐Ÿšš Free UK Delivery on orders over ยฃ150
Processing The item has been added
Enquire For Bulk Order
✓ 1-Year Performance Guarantee | SDS & Datasheet Included | Expert Tech Support | Covered by St John's Laboratory Guarantee

General Information

Short DescriptionRabbit monoclonal Ly-6A/E (Sca-1) (1-100) antibody for use in WB and ELISA in mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityMouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS9MR
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolLy6a
Gene ID110454
Uniprot IDLY6A_MOUSE
Immunogen Region1-100
Immunogen SequenceMDTSHTTKSCLLILLVALLC AERAQGLECYQCYGVPFETS CPSITCPYPDGVCVTQEAAV IVDSQTRKVKNNLCLPICPP NIESMEILGTKVNVKTSCCQ
SpecificityA synthetic peptide corresponding to a sequence within amino acids 1-100 of mouse Ly-6A/E (Sca-1) (P05533).

Additional Info

Tissue Specificity Widely expressed.
Post Translational Modifications O-glycosylated. Not N-glycosylated. Not phosphorylated.
Function T-cell activation.
Protein Name Lymphocyte Antigen 6a-2/6e-1
Ly-6a.2/Ly-6e.1
Stem Cell Antigen 1
Sca-1
T-Cell-Activating Protein
Tap
Database Links
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Alternative Antibody Names Anti-Lymphocyte Antigen 6a-2/6e-1 antibody
Anti-Ly-6a.2/Ly-6e.1 antibody
Anti-Stem Cell Antigen 1 antibody
Anti-Sca-1 antibody
Anti-T-Cell-Activating Protein antibody
Anti-Tap antibody
Anti-Ly6a antibody
Anti-Ly6 antibody

Information sourced from Uniprot.org

Citations

Product Review

!