• Western blot analysis of extracts of various cell lines, using Ly-6A/E (Sca-1) antibody (STJ11103169) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Ly-6A/E antibody (1-100) [S9MR] (STJ11103169)

SKU:
STJ11103169

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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Ly-6A/E (Sca-1) (1-100) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Ly6a
Gene ID: 110454
Uniprot ID: LY6A_MOUSE
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of mouse Ly-6A/E (Sca-1) (P05533).
Immunogen Sequence: MDTSHTTKSCLLILLVALLC AERAQGLECYQCYGVPFETS CPSITCPYPDGVCVTQEAAV IVDSQTRKVKNNLCLPICPP NIESMEILGTKVNVKTSCCQ
Tissue Specificity Widely expressed.
Post Translational Modifications O-glycosylated. Not N-glycosylated. Not phosphorylated.
Function T-cell activation.
Protein Name Lymphocyte Antigen 6a-2/6e-1
Ly-6a.2/Ly-6e.1
Stem Cell Antigen 1
Sca-1
T-Cell-Activating Protein
Tap
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Alternative Antibody Names Anti-Lymphocyte Antigen 6a-2/6e-1 antibody
Anti-Ly-6a.2/Ly-6e.1 antibody
Anti-Stem Cell Antigen 1 antibody
Anti-Sca-1 antibody
Anti-T-Cell-Activating Protein antibody
Anti-Tap antibody
Anti-Ly6a antibody
Anti-Ly6 antibody

Information sourced from Uniprot.org

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