• Immunofluorescence analysis of PC-12 cells using LTBR Rabbit polyclonal antibody (STJ27304) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of LTBR in paraffin-embedded human tonsil using LTBR Rabbit polyclonal antibody (STJ27304) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using LTBR Rabbit polyclonal antibody (STJ27304) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-LTBR antibody (336-435) (STJ27304)

SKU:
STJ27304

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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LTBR
Gene ID: 4055
Uniprot ID: TNR3_HUMAN
Immunogen Region: 336-435
Specificity: A synthetic peptide corresponding to a sequence within amino acids 336-435 of human LTBR (NP_002333.1).
Immunogen Sequence: GEQSQVAHGTNGIHVTGGSM TITGNIYIYNGPVLGGPPGP GDLPATPEPPYPIPEEGDPG PPGLSTPHQEDGKAWHLAET EHCGATPSNRGPRNQFITHD
Function Receptor for the heterotrimeric lymphotoxin containing LTA and LTB, and for TNFS14/LIGHT. Activates NF-kappa-B signaling pathway upon stimulation with lymphotoxin (LTA(1)-LTB(2)). Promotes apoptosis via TRAF3 and TRAF5. May play a role in the development of lymphoid organs.
Protein Name Tumor Necrosis Factor Receptor Superfamily Member 3
Lymphotoxin-Beta Receptor
Tumor Necrosis Factor C Receptor
Tumor Necrosis Factor Receptor 2-Related Protein
Tumor Necrosis Factor Receptor Type Iii
Tnf-Riii
Tnfr-Iii
Database Links Reactome: R-HSA-5668541
Reactome: R-HSA-5676594
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Tumor Necrosis Factor Receptor Superfamily Member 3 antibody
Anti-Lymphotoxin-Beta Receptor antibody
Anti-Tumor Necrosis Factor C Receptor antibody
Anti-Tumor Necrosis Factor Receptor 2-Related Protein antibody
Anti-Tumor Necrosis Factor Receptor Type Iii antibody
Anti-Tnf-Riii antibody
Anti-Tnfr-Iii antibody
Anti-LTBR antibody
Anti-D12S370 antibody
Anti-TNFCR antibody
Anti-TNFR3 antibody
Anti-TNFRSF3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance