• Immunofluorescence analysis of NIH-3T3 cells using LRPAP1 Rabbit polyclonal antibody (STJ24426) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of LRPAP1 in paraffin-embedded mouse brain using LRPAP1 Rabbit polyclonal antibody (STJ24426) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of LRPAP1 in paraffin-embedded rat brain using LRPAP1 Rabbit polyclonal antibody (STJ24426) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of LRPAP1 in paraffin-embedded human liver cancer using LRPAP1 Rabbit polyclonal antibody (STJ24426) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using LRPAP1 Rabbit polyclonal antibody (STJ24426) at 1:200 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-LRPAP1 antibody (100-200) (STJ24426)

SKU:
STJ24426

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:100
IF/ICC:1:50-1:100
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LRPAP1
Gene ID: 4043
Uniprot ID: AMRP_HUMAN
Immunogen Region: 100-200
Specificity: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human LRPAP1 (P30533).
Immunogen Sequence: GLDEDGEKEARLIRNLNVIL AKYGLDGKKDARQVTSNSLS GTQEDGLDDPRLEKLWHKAK TSGKFSGEELDKLWREFLHH KEKVHEYNVLLETLSRTEEI H
Post Translational Modifications N-glycosylated.
Function Molecular chaperone for LDL receptor-related proteins that may regulate their ligand binding activity along the secretory pathway.
Protein Name Alpha-2-Macroglobulin Receptor-Associated Protein
Alpha-2-Mrap
Low Density Lipoprotein Receptor-Related Protein-Associated Protein 1
Rap
Cellular Localisation Rough Endoplasmic Reticulum Lumen
Endoplasmic Reticulum-Golgi Intermediate Compartment Lumen
Golgi Apparatus
Cis-Golgi Network
Golgi Apparatus Lumen
Endosome Lumen
Cell Surface
May Be Associated With Receptors At The Cell Surface
Alternative Antibody Names Anti-Alpha-2-Macroglobulin Receptor-Associated Protein antibody
Anti-Alpha-2-Mrap antibody
Anti-Low Density Lipoprotein Receptor-Related Protein-Associated Protein 1 antibody
Anti-Rap antibody
Anti-LRPAP1 antibody
Anti-A2MRAP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance