Western blot analysis of lysates from HL-60 cells, using LPAR2 Rabbit polyclonal antibody (STJ117019) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-LPAR2 antibody (250-350) (STJ117019)

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STJ117019
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Short Description :Rabbit polyclonal LPAR2 (250-350) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents.
Applications:WB/ELISA
Host:Rabbit
Reactivity:Human
Note:STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality :Polyclonal
Isotype:IgG
Conjugation:Unconjugated
Concentration:Lot specific
Purification:Affinity purification
Dilution Range:WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Formulation:PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Storage Instruction:Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol:LPAR2
Gene ID:9170
Uniprot ID:LPAR2_HUMAN
Immunogen Region:250-350
Immunogen Sequence:FVVCWTPGQVVLLLDGLGCE SCNVLAVEKYFLLLAEANSL VNAAVYSCRDAEMRRTFRRL LCCACLRQSTRESVHYTSSA QGGASTRIMLPENGHPLMDS T
Specificity:A synthetic peptide corresponding to a sequence within amino acids 250-350 of human LPAR2 (NP_004711.2).
Tissue Specificity Expressed most abundantly in testes and peripheral blood leukocytes with less expression in pancreas, spleen, thymus and prostate. Little or no expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney, ovary, small intestine, or colon.
Function Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Seems to be coupled to the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Plays a key role in phospholipase C-beta (PLC-beta) signaling pathway. Stimulates phospholipase C (PLC) activity in a manner that is independent of RALA activation.
Protein Name Lysophosphatidic Acid Receptor 2
Lpa Receptor 2
Lpa-2
Lysophosphatidic Acid Receptor Edg-4
Database Links Reactome: R-HSA-416476
Reactome: R-HSA-418594
Reactome: R-HSA-419408
Cellular Localisation Cell Surface
Cell Membrane
Multi-Pass Membrane Protein
Prior To Lpa Treatment Found Predominantly At The Cell Surface But In The Presence Of Lpa Colocalizes With Rala In The Endocytic Vesicles
Alternative Antibody Names Anti-Lysophosphatidic Acid Receptor 2 antibody
Anti-Lpa Receptor 2 antibody
Anti-Lpa-2 antibody
Anti-Lysophosphatidic Acid Receptor Edg-4 antibody
Anti-LPAR2 antibody
Anti-EDG4 antibody
Anti-LPA2 antibody

Information sourced from Uniprot.org

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