• Immunoprecipitation analysis of 300 Mu g extracts of Jurkat cells using 3 Mu g LOX antibody (STJ11101349). Western blot was performed from the immunoprecipitate using LOX antibody (STJ11101349) at a dilution of 1:1000.
  • Immunohistochemistry analysis of LOX in paraffin-embedded rat heart tissue using LOX Rabbit monoclonal antibody (STJ11101349) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of LOX in paraffin-embedded mouse kidney tissue using LOX Rabbit monoclonal antibody (STJ11101349) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of LOX in paraffin-embedded human breast tissue using LOX Rabbit monoclonal antibody (STJ11101349) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of LOX in paraffin-embedded rat kidney using LOX Rabbit monoclonal antibody (STJ11101349) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of LOX in paraffin-embedded human colon using LOX Rabbit monoclonal antibody (STJ11101349) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using LOX Rabbit monoclonal antibody (STJ11101349) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-LOX antibody (318-417) [S9MR] (STJ11101349)

SKU:
STJ11101349

€183.13 - €799.38
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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-LOX (318-417) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LOX
Gene ID: 4015
Uniprot ID: LYOX_HUMAN
Immunogen Region: 318-417
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 318-417 of human LOX (P28300).
Immunogen Sequence: GHKASFCLEDTSCDYGYHRR FACTAHTQGLSPGCYDTYGA DIDCQWIDITDVKPGNYILK VSVNPSYLVPESDYTNNVVR CDIRYTGHHAYASGCTISPY
Tissue Specificity Heart, placenta, skeletal muscle, kidney, lung and pancreas.
Post Translational Modifications The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine. Proteolytically cleaved by BMP1 which removes the propeptide. Also proteolytically cleaved by ADAMTS2 and ADAMTS14, but not by ADAMTS3, at an additional cleavage site downstream of the BMP1 cleavage site. The propeptide plays a role in directing the deposition of this enzyme to elastic fibers, via interaction with tropoelastin. Cleavage by BMP1 to remove the propeptide does not increase enzymatic activity but increases binding to collagen. Cleavage by ADAMTS2 produces a form with reduced collagen-binding activity. Sulfated at Tyr-187 and also at either Tyr-183 or Tyr-184 which enhances binding to collagen.
Function Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. Regulator of Ras expression. May play a role in tumor suppression. Plays a role in the aortic wall architecture.
Protein Name Protein-Lysine 6-Oxidase
Lysyl Oxidase Cleaved Into - Protein-Lysine 6-Oxidase - Long Form - Protein-Lysine 6-Oxidase - Short Form
Database Links Reactome: R-HSA-1566948
Reactome: R-HSA-2243919
Cellular Localisation Secreted
Extracellular Space
Alternative Antibody Names Anti-Protein-Lysine 6-Oxidase antibody
Anti-Lysyl Oxidase Cleaved Into - Protein-Lysine 6-Oxidase - Long Form - Protein-Lysine 6-Oxidase - Short Form antibody
Anti-LOX antibody

Information sourced from Uniprot.org

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