• Western blot analysis of extracts of various cell lines, using Lamin B2 rabbit monoclonal antibody (STJ11102218) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using Lamin B2 rabbit monoclonal antibody (STJ11102218) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Lamin B2 rabbit monoclonal antibody (STJ11102218) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Lamin B2 rabbit monoclonal antibody (STJ11102218) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Lamin B2 in paraffin-embedded human colon carcinoma tissue using Lamin B2 rabbit monoclonal antibody (A22347) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Lamin B2 in paraffin-embedded human spleen tissue using Lamin B2 rabbit monoclonal antibody (A22347) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Lamin B2 in paraffin-embedded mouse brain tissue using Lamin B2 rabbit monoclonal antibody (A22347) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Lamin B2 in paraffin-embedded mouse kidney tissue using Lamin B2 rabbit monoclonal antibody (A22347) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Lamin B2 in paraffin-embedded mouse liver tissue using Lamin B2 rabbit monoclonal antibody (A22347) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Confocal imaging of HeLa cells using Lamin B2 rabbit monoclonal antibody (STJ11102218, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin rabbit monoclonal antibody (dilution 1:100) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

Anti-LMNB2 antibody (521-620) [S8MR] (STJ11102218)

SKU:
STJ11102218

£129.50 - £574.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LMNB2
Gene ID: 84823
Uniprot ID: LMNB2_HUMAN
Immunogen Region: 521-620
Specificity: A synthetic peptide corresponding to a sequence within amino acids 521-620 of human Lamin B2 (Q03252).
Immunogen Sequence: YILRAGQMVTVWAAGAGVAH SPPSTLVWKGQSSWGTGESF RTVLVNADGEEVAMRTVKKS SVMRENENGEEEEEEAEFGE EDLFHQQGDPRTTSRGCYVM
Post Translational Modifications B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. Phosphorylation plays a key role in lamin organization, subcellular localization and nuclear envelope disintegration. Phosphorylation by CDK1 at Ser-37 and Ser-407 at the onset of mitosis drives lamin disassembly and nuclear envelope breakdown.
Function Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane. Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively.
Protein Name Lamin-B2
Cellular Localisation Nucleus Lamina
Alternative Antibody Names Anti-Lamin-B2 antibody
Anti-LMNB2 antibody
Anti-LMN2 antibody

Information sourced from Uniprot.org

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