| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | LIPE |
| Gene ID: | 3991 |
| Uniprot ID: | LIPS_HUMAN |
| Immunogen Region: | 900-1000 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 900-1000 of human LIPE (NP_005348.2). |
| Immunogen Sequence: | TLSPSTPSDVNFLLPPEDAG EEAEAKNELSPMDRGLGVRA AFPEGFHPRRSSQGATQMPL YSSPIVKNPFMSPLLAPDSM LKSLPPVHIVACALDPMLDD S |
| Tissue Specificity | Testis. |
| Post Translational Modifications | Phosphorylation by AMPK reduces its translocation towards the lipid droplets. |
| Function | Lipase with broad substrate specificity, catalyzing the hydrolysis of triacylglycerols (TAGs), diacylglycerols (DAGs), monoacylglycerols (MAGs), cholesteryl esters and retinyl esters. Shows a preferential hydrolysis of DAGs over TAGs and MAGs and preferentially hydrolyzes the fatty acid (FA) esters at the sn-3 position of the glycerol backbone in DAGs. Preferentially hydrolyzes FA esters at the sn-1 and sn-2 positions of the glycerol backbone in TAGs. Catalyzes the hydrolysis of 2-arachidonoylglycerol, an endocannabinoid and of 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. |
| Protein Name | Hormone-Sensitive Lipase Hsl Monoacylglycerol Lipase Lipe Retinyl Ester Hydrolase Reh |
| Database Links | Reactome: R-HSA-163560 |
| Cellular Localisation | Cell Membrane Membrane Caveola Cytoplasm Cytosol Lipid Droplet Found In The High-Density Caveolae Translocates To The Cytoplasm From The Caveolae Upon Insulin Stimulation Phosphorylation By Ampk Reduces Its Translocation Towards The Lipid Droplets |
| Alternative Antibody Names | Anti-Hormone-Sensitive Lipase antibody Anti-Hsl antibody Anti-Monoacylglycerol Lipase Lipe antibody Anti-Retinyl Ester Hydrolase antibody Anti-Reh antibody Anti-LIPE antibody |
Information sourced from Uniprot.org