• Immunofluorescence analysis of paraffin-embedded mouse intestine using TRPA1 Rabbit polyclonal antibody (STJ114418) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Rat colon using TRPA1 Rabbit polyclonal antibody (STJ114418) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-TRPA1 antibody (900-1000) (STJ114418)

SKU:
STJ114418

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TRPA1 (900-1000) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TRPA1
Gene ID: 8989
Uniprot ID: TRPA1_HUMAN
Immunogen Region: 900-1000
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 900-1000 of human TRPA1 (NP_015628.2).
Immunogen Sequence: SPLLSIIQTFSMMLGDINYR ESFLEPYLRNELAHPVLSFA QLVSFTIFVPIVLMNLLIGL AVGDIAEVQKHASLKRIAMQ VELHTSLEKKLPLWFLRKVD Q
Tissue Specificity Expressed at very low levels in fibroblasts.
Post Translational Modifications TRPA1 activation by electrophiles occurs though covalent modification of specific cysteine residues in the N-terminal cytoplasmic domain. Hydroxylation is required for TRPA1 activity inhibition in normoxia. In hypoxia, the decrease in oxygen concentration diminishes the activity of the hydroxylase EGLN1, thus relieving TRPA1 from inhibition and ultimately leading to channel activation. Oxidation of Cys-633 and Cys-856 in hyperoxia may override the hydroxylase EGLN1-mediated inhibition, causing TRPA1 activation.
Function Receptor-activated non-selective cation channel involved in pain detection and possibly also in cold perception, oxygen concentration perception, cough, itch, and inner ear function. Shows 8-fold preference for divalent over monovalent cations. Has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of irritants, such as allylthiocyanate (AITC) from mustard oil or wasabi, cinnamaldehyde, diallyl disulfide (DADS) from garlic, and acrolein, an irritant from tears gas and vehicle exhaust fumes. Acts also as an ionotropic cannabinoid receptor by being activated by delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana. Is activated by a large variety of structurally unrelated electrophilic and non-electrophilic chemical compounds. Electrophilic ligands activate TRPA1 by interacting with critical N-terminal Cys residues in a covalent manner, whereas mechanisms of non-electrophilic ligands are not well determined. May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Probably operated by a phosphatidylinositol second messenger system.
Protein Name Transient Receptor Potential Cation Channel Subfamily A Member 1
Ankyrin-Like With Transmembrane Domains Protein 1
Transformation-Sensitive Protein P120
P120
Wasabi Receptor
Database Links Reactome: R-HSA-3295583
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Transient Receptor Potential Cation Channel Subfamily A Member 1 antibody
Anti-Ankyrin-Like With Transmembrane Domains Protein 1 antibody
Anti-Transformation-Sensitive Protein P120 antibody
Anti-P120 antibody
Anti-Wasabi Receptor antibody
Anti-TRPA1 antibody
Anti-ANKTM1 antibody

Information sourced from Uniprot.org

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