| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | LIMS1 |
| Gene ID: | 3987 |
| Uniprot ID: | LIMS1_HUMAN |
| Immunogen Region: | 1-325 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-325 of human LIMS1 (NP_001180417.1). |
| Immunogen Sequence: | MANALASATCERCKGGFAPA EKIVNSNGELYHEQCFVCAQ CFQQFPEGLFYEFEGRKYCE HDFQMLFAPCCHQCGEFIIG RVIKAMNNSWHPECFRCDLC QEVLADIGFVKNAGRHLCRP CHNREKARGLGKYICQKCHA IIDEQPLIFKNDPYHPDHFN CANCGKELTADARELKGELY CLPCHDKMGVPICGACRRPI EGRVVNAMGKQWHVEHFVCA KCEKPFLGHRHYERKGLAY |
| Tissue Specificity | Expressed in most tissues except in the brain. |
| Function | Within the IPP (ILK-PINCH-PARVIN) complex, binds to F-actin, promoting F-actin bundling, a process required to generate force for actin cytoskeleton reorganization and subsequent dynamic cell adhesion events such as cell spreading and migration. |
| Protein Name | Lim And Senescent Cell Antigen-Like-Containing Domain Protein 1Particularly Interesting New Cys-His Protein 1Pinch-1Renal Carcinoma Antigen Ny-Ren-48 |
| Database Links | Reactome: R-HSA-446353Reactome: R-HSA-446388 |
| Cellular Localisation | Cell JunctionFocal AdhesionCell MembranePeripheral Membrane ProteinCytoplasmic Side |
| Alternative Antibody Names | Anti-Lim And Senescent Cell Antigen-Like-Containing Domain Protein 1 antibodyAnti-Particularly Interesting New Cys-His Protein 1 antibodyAnti-Pinch-1 antibodyAnti-Renal Carcinoma Antigen Ny-Ren-48 antibodyAnti-LIMS1 antibodyAnti-PINCH antibodyAnti-PINCH1 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and AKR1A1 knockout (KO) 293T cells, using [KO Validated] AKR1A1 Rabbit polyclonal antibody (STJ119987) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/98772/382820/STJ119987_1__54161.1713151317.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and AKR1A1 knockout (KO) 293T cells, using [KO Validated] AKR1A1 Rabbit polyclonal antibody (STJ119987) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
