• Western blot analysis of extracts of various cell lines, using LHX4 Antibody (STJ110375) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded mouse cancer using LHX4 antibody (STJ110375) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human kidney cancer using LHX4 antibody (STJ110375) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human kidney cancer using LHX4 antibody (STJ110375) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse cancer using LHX4 antibody (STJ110375) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using LHX4 Antibody (STJ110375) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-LHX4 antibody (121-390) (STJ110375)

SKU:
STJ110375

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LHX4 (121-390) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LHX4
Gene ID: 89884
Uniprot ID: LHX4_HUMAN
Immunogen Region: 121-390
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 121-390 of human LHX4 (NP_203129.1).
Immunogen Sequence: NRQLATGDEFYLMEDGRLVC KEDYETAKQNDDSEAGAKRP RTTITAKQLETLKNAYKNSP KPARHVREQLSSETGLDMRV VQVWFQNRRAKEKRLKKDAG RHRWGQFYKSVKRSRGSSKQ EKESSAEDCGVSDSELSFRE DQILSELGHTNRIYGNVGDV TGGQLMNGSFSMDGTGQSYQ DLRDGSPYGIPQSPSSISSL PSHAPLLNGLDYTVDSNLGI IAHAGQGVSQTLRAMAGGP
Function May play a critical role in the development of respiratory control mechanisms and in the normal growth and maturation of the lung. Binds preferentially to methylated DNA.
Protein Name Lim/Homeobox Protein Lhx4
Lim Homeobox Protein 4
Database Links Reactome: R-HSA-9010553
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Lim/Homeobox Protein Lhx4 antibody
Anti-Lim Homeobox Protein 4 antibody
Anti-LHX4 antibody

Information sourced from Uniprot.org

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