• Immunoprecipitation analysis of 150 Mu g extracts of Jurkat cells using 3 Mu g L-Plastin/LCP1 antibody (STJ27507). Western blot was performed from the immunoprecipitate using L-Plastin/LCP1 antibody (STJ27507) at a dilution of 1:1000.
  • Western blot analysis of various lysates using L-Plastin/LCP1 Rabbit polyclonal antibody (STJ27507) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-LCP1 antibody (1-270) (STJ27507)

SKU:
STJ27507

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LCP1 (1-270) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:20-1:50
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LCP1
Gene ID: 3936
Uniprot ID: PLSL_HUMAN
Immunogen Region: 1-270
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human L-Plastin/LCP1 (NP_002289.2).
Immunogen Sequence: MARGSVSDEEMMELREAFAK VDTDGNGYISFNELNDLFKA ACLPLPGYRVREITENLMAT GDLDQDGRISFDEFIKIFHG LKSTDVAKTFRKAINKKEGI CAIGGTSEQSSVGTQHSYSE EEKYAFVNWINKALENDPDC RHVIPMNPNTNDLFNAVGDG IVLCKMINLSVPDTIDERTI NKKKLTPFTIQENLNLALNS ASAIGCHVVNIGAEDLKEGK PYLVLGLLWQVIKIGLFAD
Tissue Specificity Detected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T-lymphocytes, neutrophils, monocytes, B-lymphocytes, and myeloid cells.
Post Translational Modifications Phosphorylated on a serine residue in response to costimulation through TCR/CD3 and CD2 or CD28. Serine phosphorylation promotes association with the actin cytoskeleton and targeting to peripheral cell projections.
Function Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.
Protein Name Plastin-2
L-Plastin
Lc64p
Lymphocyte Cytosolic Protein 1
Lcp-1
Database Links Reactome: R-HSA-8950505
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Junction
Cell Projection
Ruffle Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Relocalizes To The Immunological Synapse Between Peripheral Blood T-Lymphocytes And Antibody-Presenting Cells In Response To Costimulation Through Tcr/Cd3 And Cd2 Or Cd28
Associated With The Actin Cytoskeleton At Membrane Ruffles
Relocalizes To Actin-Rich Cell Projections Upon Serine Phosphorylation
Alternative Antibody Names Anti-Plastin-2 antibody
Anti-L-Plastin antibody
Anti-Lc64p antibody
Anti-Lymphocyte Cytosolic Protein 1 antibody
Anti-Lcp-1 antibody
Anti-LCP1 antibody
Anti-PLS2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance