• Immunofluorescence analysis of U2OS cells using LARS Rabbit polyclonal antibody (STJ117090) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using LARS Rabbit polyclonal antibody (STJ117090) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using LARS Rabbit polyclonal antibody (STJ117090) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-LARS antibody (1-270) (STJ117090)

SKU:
STJ117090

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LARS (1-270) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LARS1
Gene ID: 51520
Uniprot ID: SYLC_HUMAN
Immunogen Region: 1-270
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human LARS (NP_064502.9).
Immunogen Sequence: MAERKGTAKVDFLKKIEKEI QQKWDTERVFEVNASNLEKQ TSKGKYFVTFPYPYMNGRLH LGHTFSLSKCEFAVGYQRLK GKCCLFPFGLHCTGMPIKAC ADKLKREIELYGCPPDFPDE EEEEEETSVKTEDIIIKDKA KGKKSKAAAKAGSSKYQWGI MKSLGLSDEEIVKFSEAEHW LDYFPPLAIQDLKRMGLKVD WRRSFITTDVNPYYDSFVRW QFLTLRERNKIKFGKRYTI
Function Catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Exhibits a post-transfer editing activity to hydrolyze mischarged tRNAs.
Protein Name Leucine--Trna Ligase - Cytoplasmic
Leucyl-Trna Synthetase
Leurs
Database Links Reactome: R-HSA-2408522
Reactome: R-HSA-379716
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Leucine--Trna Ligase - Cytoplasmic antibody
Anti-Leucyl-Trna Synthetase antibody
Anti-Leurs antibody
Anti-LARS1 antibody
Anti-KIAA1352 antibody
Anti-LARS antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance