• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma (A) , lung cancer (B) and ovarian cancer tissue (C) , showing membrane and cytoplasmic localization with DAB staining using Cytokeratin 8 monoclonal antibody.
  • Confocal immunofluorescence analysis of methanol-fixed ECA109 cells (left) and HepG2 cells (right) using Cytokeratin 8 monoclonal antibody (green) , showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.
  • Western blot analysis using Cytokeratin 8 monoclonal antibody against A549 (1) , HeLa (2) , MCF-7 (3) , A431 (4) and HepG2 (5) cell lysate.

Anti-KRT8 antibody (391-483 aa) [8A5D12] (STJ97992)

SKU:
STJ97992

£139.00 - £336.00
Current Stock:
Host: Mouse
Applications: WB/IHC/IF/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Keratin, type II cytoskeletal 8 (391-483 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Monoclonal
Clone ID: 8A5D12
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRT8
Gene ID: 3856
Uniprot ID: K2C8_HUMAN
Immunogen Region: 391-483 aa
Specificity: Cytokeratin 8 Monoclonal Antibody detects endogenous levels of Cytokeratin 8 protein.
Immunogen: Purified recombinant fragment of human Cytokeratin 8 (aa391-483) expressed in E. Coli.
Post Translational Modifications Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization. O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
Function Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Protein Name Keratin - Type Ii Cytoskeletal 8
Cytokeratin-8
Ck-8
Keratin-8
K8
Type-Ii Keratin Kb8
Database Links Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Cellular Localisation Cytoplasm
Nucleus
Nucleoplasm
Nucleus Matrix
Alternative Antibody Names Anti-Keratin - Type Ii Cytoskeletal 8 antibody
Anti-Cytokeratin-8 antibody
Anti-Ck-8 antibody
Anti-Keratin-8 antibody
Anti-K8 antibody
Anti-Type-Ii Keratin Kb8 antibody
Anti-KRT8 antibody
Anti-CYK8 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance