• A431 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CK14 (ABT049) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: A431Predicted band size: 53kDaObserved band size: 53kDa
  • Human cervical squamous cell carcinoma tissue was stained with Anti-Cytokeratin 14 (ABT049) Antibody
  • Human prostate tissue was stained with Anti-Cytokeratin 14 (ABT049) Antibody
  • Human tonsil tissue was stained with Anti-Cytokeratin 14 (ABT049) Antibody

Anti-KRT14 antibody (400-472 aa) [PT1674] (STJ196754)

SKU:
STJ196754

£168.00 - £226.00
Current Stock:
Host: Mouse
Applications: IHC/WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Keratin, type I cytoskeletal 14 (400-472 aa) is suitable for use in Immunohistochemistry, Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: PT1674
Conjugation: Unconjugated
Isotype: IgG1k
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: WB 500-2000
IHC-P 1:100-500
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRT14
Gene ID: 3861
Uniprot ID: K1C14_HUMAN
Immunogen Region: 400-472 aa
Specificity: This antibody detects endogenous levels of human Cytokeratin 14. Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH8.0 was highly recommended as antigen repair method in paraffin section. The antib
Immunogen: Synthesized peptide derived from the human Cytokeratin 14 at the amino acid range 400-472
Post Translational Modifications A disulfide bond is formed between rather than within filaments and promotes the formation of a keratin filament cage around the nucleus. Ubiquitinated by the BCR(KLHL24) E3 ubiquitin ligase complex.
Function The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
Protein Name Keratin - Type I Cytoskeletal 14
Cytokeratin-14
Ck-14
Keratin-14
K14
Database Links Reactome: R-HSA-446107
Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Cellular Localisation Cytoplasm
Nucleus
Expressed In Both As A Filamentous Pattern
Alternative Antibody Names Anti-Keratin - Type I Cytoskeletal 14 antibody
Anti-Cytokeratin-14 antibody
Anti-Ck-14 antibody
Anti-Keratin-14 antibody
Anti-K14 antibody
Anti-KRT14 antibody

Information sourced from Uniprot.org

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