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Anti-KRAS antibody (30-189) (STJ114577)

Western blot analysis of extracts from normal (control) and KRAS knockout (KO) 293T cells, using KRAS antibody (STJ114577) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Western blot analysis of various lysates, using KRAS antibody (STJ114577) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunofluorescence analysis of L929 cells using [KO Validated] KRAS rabbit polyclonal antibody (STJ114577) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.

Anti-KRAS antibody (30-189) (STJ114577)

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STJ114577

£129.50 - £639.50

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Rabbit
Applications:	WB/IF
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-KRAS (30-189) is suitable for use in Western Blot and Immunofluorescence research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:1000-1:5000 IF/ICC 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	KRAS
Gene ID:	3845
Uniprot ID:	RASK_HUMAN
Immunogen Region:	30-189
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-189 of human KRAS (NP_203524.1).
Immunogen Sequence:	DEYDPTIEDSYRKQVVIDGE TCLLDILDTAGQEEYSAMRD QYMRTGEGFLCVFAINNTKS FEDIHHYREQIKRVKDSEDV PMVLVGNKCDLPSRTVDTKQ AQDLARSYGIPFIETSAKTR QRVEDAFYTLVREIRQYRLK KISKEEKTPGCVKIKKCII

Post Translational Modifications	Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs). Palmitoylated at Lys-182, Lys-184 and Lys-185. Palmitoylation on lysine residues is promoted by palmitoylation at Cys-180. Lysine-depalmitoylation by SIRT2 promotes its localization to endomembranes in endocytic pathways. Ubiquitinated by the BCR(LZTR1) E3 ubiquitin ligase complex at Lys-170 in a non-degradative manner, leading to inhibit Ras signaling by decreasing Ras association with membranes. (Microbial infection) Glucosylated at Thr-35 by P.sordellii toxin TcsL.
Function	Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation. Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner.
Protein Name	Gtpase Kras K-Ras 2 Ki-Ras C-K-Ras C-Ki-Ras Cleaved Into - Gtpase Kras - N-Terminally Processed
Database Links	Reactome: R-HSA-112412 Reactome: R-HSA-1169092 Reactome: R-HSA-1236382 Reactome: R-HSA-1250196 Reactome: R-HSA-1250347 Reactome: R-HSA-1433557 Reactome: R-HSA-167044 Reactome: R-HSA-171007 Reactome: R-HSA-179812 Reactome: R-HSA-180336 Reactome: R-HSA-186763 Reactome: R-HSA-1963640 Reactome: R-HSA-210993 Reactome: R-HSA-2179392 Reactome: R-HSA-2424491 Reactome: R-HSA-2428933 Reactome: R-HSA-2871796 Reactome: R-HSA-375165 Reactome: R-HSA-4086398 P01116-2 Reactome: R-HSA-442982 Reactome: R-HSA-5218921 Reactome: R-HSA-5621575 Reactome: R-HSA-5637810 Reactome: R-HSA-5654688 Reactome: R-HSA-5654693 Reactome: R-HSA-5654699 Reactome: R-HSA-5654700 Reactome: R-HSA-5654704 Reactome: R-HSA-5654706 Reactome: R-HSA-5654712 Reactome: R-HSA-5654719 Reactome: R-HSA-5655253 Reactome: R-HSA-5655291 Reactome: R-HSA-5655302 Reactome: R-HSA-5655332 Reactome: R-HSA-5658442 Reactome: R-HSA-5673000 Reactome: R-HSA-5673001 Reactome: R-HSA-5674135 Reactome: R-HSA-5675221 Reactome: R-HSA-6802946 Reactome: R-HSA-6802948 Reactome: R-HSA-6802952 Reactome: R-HSA-6802953 Reactome: R-HSA-6802955 Reactome: R-HSA-74751 Reactome: R-HSA-8849471 Reactome: R-HSA-8851805 Reactome: R-HSA-8951936 Reactome: R-HSA-9026519 Reactome: R-HSA-9027284 Reactome: R-HSA-9028731 Reactome: R-HSA-9034864 Reactome: R-HSA-9607240 Reactome: R-HSA-9634285 Reactome: R-HSA-9634635 Reactome: R-HSA-9648002 Reactome: R-HSA-9649948 Reactome: R-HSA-9656223 Reactome: R-HSA-9664565 Reactome: R-HSA-9665348 Reactome: R-HSA-9665686 Reactome: R-HSA-9670439 Reactome: R-HSA-9673767 Reactome: R-HSA-9673770 Reactome: R-HSA-9674555 Reactome: R-HSA-9680350 Reactome: R-HSA-9703465 Reactome: R-HSA-9703648 Reactome: R-HSA-9753510 Reactome: R-HSA-9753512
Cellular Localisation	Cell Membrane Lipid-Anchor Cytoplasmic Side Endomembrane System Cytoplasm Cytosol Isoform 2b: Cell Membrane
Alternative Antibody Names	Anti-Gtpase Kras antibody Anti-K-Ras 2 antibody Anti-Ki-Ras antibody Anti-C-K-Ras antibody Anti-C-Ki-Ras Cleaved Into - Gtpase Kras - N-Terminally Processed antibody Anti-KRAS antibody Anti-KRAS2 antibody Anti-RASK2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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