| Host: |
Mouse |
| Applications: |
WB/IHC/IF/ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Histone-lysine N-methyltransferase 2A (3751-3968 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
10F8D7 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1 |
| Formulation: |
Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC 1:200-1:1000ELISA 1:10000IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
KMT2A |
| Gene ID: |
4297 |
| Uniprot ID: |
KMT2A_HUMAN |
| Immunogen Region: |
3751-3968 aa |
| Specificity: |
MLL Monoclonal Antibody detects endogenous levels of MLL protein. |
| Immunogen: |
Purified recombinant fragment of MLL (aa3751-3968) expressed in E. Coli. |
| Post Translational Modifications | Proteolytic cleavage by TASP1 generates MLL cleavage product N320 and MLL cleavage product C180, which reassemble through a non-covalent association. 2 cleavage sites exist, cleavage site 1 (CS1) and cleavage site 2 (CS2), to generate MLL cleavage products N320 and C180. CS2 is the major site. Phosphorylation increases its interaction with PSIP1. Auto-methylated at Cys-3882: auto-methylation is inhibited by the WRAD complex and unmodified histone H3. |
| Function | Histone methyltransferase that plays an essential role in early development and hematopoiesis. Catalytic subunit of the MLL1/MLL complex, a multiprotein complex that mediates both methylation of 'Lys-4' of histone H3 (H3K4me) complex and acetylation of 'Lys-16' of histone H4 (H4K16ac). Catalyzes methyl group transfer from S-adenosyl-L-methionine to the epsilon-amino group of 'Lys-4' of histone H3 (H3K4) via a non-processive mechanism. Part of chromatin remodeling machinery predominantly forms H3K4me1 and H3K4me2 methylation marks at active chromatin sites where transcription and DNA repair take place. Has weak methyltransferase activity by itself, and requires other component of the MLL1/MLL complex to obtain full methyltransferase activity. Has no activity toward histone H3 phosphorylated on 'Thr-3', less activity toward H3 dimethylated on 'Arg-8' or 'Lys-9', while it has higher activity toward H3 acetylated on 'Lys-9'. Binds to unmethylated CpG elements in the promoter of target genes and helps maintain them in the nonmethylated state. Required for transcriptional activation of HOXA9. Promotes PPP1R15A-induced apoptosis. Plays a critical role in the control of circadian gene expression and is essential for the transcriptional activation mediated by the CLOCK-BMAL1 heterodimer. Establishes a permissive chromatin state for circadian transcription by mediating a rhythmic methylation of 'Lys-4' of histone H3 (H3K4me) and this histone modification directs the circadian acetylation at H3K9 and H3K14 allowing the recruitment of CLOCK-BMAL1 to chromatin. Also has auto-methylation activity on Cys-3882 in absence of histone H3 substrate. |
| Protein Name | Histone-Lysine N-Methyltransferase 2aLysine N-Methyltransferase 2aAll-1Cxxc-Type Zinc Finger Protein 7Cysteine Methyltransferase Kmt2aMyeloid/Lymphoid Or Mixed-Lineage LeukemiaMyeloid/Lymphoid Or Mixed-Lineage Leukemia Protein 1Trithorax-Like ProteinZinc Finger Protein Hrx Cleaved Into - Mll Cleavage Product N320N-Terminal Cleavage Product Of 320 KdaP320 - Mll Cleavage Product C180C-Terminal Cleavage Product Of 180 KdaP180 |
| Database Links | Reactome: R-HSA-3214841Reactome: R-HSA-8936459Reactome: R-HSA-8939236Reactome: R-HSA-9616222Reactome: R-HSA-9772755 |
| Cellular Localisation | NucleusMll Cleavage Product N320: NucleusMll Cleavage Product C180: NucleusLocalizes To A Diffuse Nuclear Pattern When Not Associated With Mll Cleavage Product N320 |
| Alternative Antibody Names | Anti-Histone-Lysine N-Methyltransferase 2a antibodyAnti-Lysine N-Methyltransferase 2a antibodyAnti-All-1 antibodyAnti-Cxxc-Type Zinc Finger Protein 7 antibodyAnti-Cysteine Methyltransferase Kmt2a antibodyAnti-Myeloid/Lymphoid Or Mixed-Lineage Leukemia antibodyAnti-Myeloid/Lymphoid Or Mixed-Lineage Leukemia Protein 1 antibodyAnti-Trithorax-Like Protein antibodyAnti-Zinc Finger Protein Hrx Cleaved Into - Mll Cleavage Product N320 antibodyAnti-N-Terminal Cleavage Product Of 320 Kda antibodyAnti-P320 - Mll Cleavage Product C180 antibodyAnti-C-Terminal Cleavage Product Of 180 Kda antibodyAnti-P180 antibodyAnti-KMT2A antibodyAnti-ALL1 antibodyAnti-CXXC7 antibodyAnti-HRX antibodyAnti-HTRX antibodyAnti-MLL antibodyAnti-MLL1 antibodyAnti-TRX1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
