| Host: | Mouse |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-Histone-lysine N-methyltransferase 2A (3751-3968 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Monoclonal |
| Clone ID : | 10F8D7 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Formulation: | Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol. |
| Purification: | Affinity purification |
| Dilution Range: | WB 1:500-1:2000 IHC 1:200-1:1000 ELISA 1:10000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | KMT2A |
| Gene ID: | 4297 |
| Uniprot ID: | KMT2A_HUMAN |
| Immunogen Region: | 3751-3968 aa |
| Specificity: | MLL Monoclonal Antibody detects endogenous levels of MLL protein. |
| Immunogen: | Purified recombinant fragment of MLL (aa3751-3968) expressed in E. Coli. |
| Post Translational Modifications | Proteolytic cleavage by TASP1 generates MLL cleavage product N320 and MLL cleavage product C180, which reassemble through a non-covalent association. 2 cleavage sites exist, cleavage site 1 (CS1) and cleavage site 2 (CS2), to generate MLL cleavage products N320 and C180. CS2 is the major site. Phosphorylation increases its interaction with PSIP1. Auto-methylated at Cys-3882: auto-methylation is inhibited by the WRAD complex and unmodified histone H3. |
| Function | Histone methyltransferase that plays an essential role in early development and hematopoiesis. Catalytic subunit of the MLL1/MLL complex, a multiprotein complex that mediates both methylation of 'Lys-4' of histone H3 (H3K4me) complex and acetylation of 'Lys-16' of histone H4 (H4K16ac). Catalyzes methyl group transfer from S-adenosyl-L-methionine to the epsilon-amino group of 'Lys-4' of histone H3 (H3K4) via a non-processive mechanism. Part of chromatin remodeling machinery predominantly forms H3K4me1 and H3K4me2 methylation marks at active chromatin sites where transcription and DNA repair take place. Has weak methyltransferase activity by itself, and requires other component of the MLL1/MLL complex to obtain full methyltransferase activity. Has no activity toward histone H3 phosphorylated on 'Thr-3', less activity toward H3 dimethylated on 'Arg-8' or 'Lys-9', while it has higher activity toward H3 acetylated on 'Lys-9'. Binds to unmethylated CpG elements in the promoter of target genes and helps maintain them in the nonmethylated state. Required for transcriptional activation of HOXA9. Promotes PPP1R15A-induced apoptosis. Plays a critical role in the control of circadian gene expression and is essential for the transcriptional activation mediated by the CLOCK-BMAL1 heterodimer. Establishes a permissive chromatin state for circadian transcription by mediating a rhythmic methylation of 'Lys-4' of histone H3 (H3K4me) and this histone modification directs the circadian acetylation at H3K9 and H3K14 allowing the recruitment of CLOCK-BMAL1 to chromatin. Also has auto-methylation activity on Cys-3882 in absence of histone H3 substrate. |
| Protein Name | Histone-Lysine N-Methyltransferase 2a Lysine N-Methyltransferase 2a All-1 Cxxc-Type Zinc Finger Protein 7 Cysteine Methyltransferase Kmt2a Myeloid/Lymphoid Or Mixed-Lineage Leukemia Myeloid/Lymphoid Or Mixed-Lineage Leukemia Protein 1 Trithorax-Like Protein Zinc Finger Protein Hrx Cleaved Into - Mll Cleavage Product N320 N-Terminal Cleavage Product Of 320 Kda P320 - Mll Cleavage Product C180 C-Terminal Cleavage Product Of 180 Kda P180 |
| Database Links | Reactome: R-HSA-3214841 Reactome: R-HSA-8936459 Reactome: R-HSA-8939236 Reactome: R-HSA-9616222 Reactome: R-HSA-9772755 |
| Cellular Localisation | Nucleus Mll Cleavage Product N320: Nucleus Mll Cleavage Product C180: Nucleus Localizes To A Diffuse Nuclear Pattern When Not Associated With Mll Cleavage Product N320 |
| Alternative Antibody Names | Anti-Histone-Lysine N-Methyltransferase 2a antibody Anti-Lysine N-Methyltransferase 2a antibody Anti-All-1 antibody Anti-Cxxc-Type Zinc Finger Protein 7 antibody Anti-Cysteine Methyltransferase Kmt2a antibody Anti-Myeloid/Lymphoid Or Mixed-Lineage Leukemia antibody Anti-Myeloid/Lymphoid Or Mixed-Lineage Leukemia Protein 1 antibody Anti-Trithorax-Like Protein antibody Anti-Zinc Finger Protein Hrx Cleaved Into - Mll Cleavage Product N320 antibody Anti-N-Terminal Cleavage Product Of 320 Kda antibody Anti-P320 - Mll Cleavage Product C180 antibody Anti-C-Terminal Cleavage Product Of 180 Kda antibody Anti-P180 antibody Anti-KMT2A antibody Anti-ALL1 antibody Anti-CXXC7 antibody Anti-HRX antibody Anti-HTRX antibody Anti-MLL antibody Anti-MLL1 antibody Anti-TRX1 antibody |
Information sourced from Uniprot.org