• Immunohistochemistry analysis of paraffin-embedded rat kidney using KLK1 Rabbit polyclonal antibody (STJ24329) at dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using KLK1 Rabbit polyclonal antibody (STJ24329) at dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using KLK1 Rabbit polyclonal antibody (STJ24329) at dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using KLK1 antibody (STJ24329) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-KLK1 antibody (25-262) (STJ24329)

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STJ24329

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KLK1 (25-262) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KLK1
Gene ID: 3816
Uniprot ID: KLK1_HUMAN
Immunogen Region: 25-262
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-262 of human KLK1 (NP_002248.1).
Immunogen Sequence: IVGGWECEQHSQPWQAALYH FSTFQCGGILVHRQWVLTAA HCISDNYQLWLGRHNLFDDE NTAQFVHVSESFPHPGFNMS LLENHTRQADEDYSHDLMLL RLTEPADTITDAVKVVELPT EEPEVGSTCLASGWGSIEPE NFSFPDDLQCVDLKILPNDE CKKAHVQKVTDFMLCVGHLE GGKDTCVGDSGGPLMCDGVL QGVTSWGYVPCGTPNKPSVA VRVLSYVKWIEDTIAENS
Tissue Specificity Isoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84.
Post Translational Modifications The O-linked polysaccharides on Ser-93, Ser-104 and Ser-167 are probably the mucin type linked to GalNAc. In PubMed:3163150, GalNAc was detected with the corresponding peptides but not located.
Function Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. (Microbial infection) Cleaves Neisseria meningitidis NHBA in saliva.Neisseria is an obligate commensal of the nasopharyngeal mucosa.
Protein Name Kallikrein-1
Kidney/Pancreas/Salivary Gland Kallikrein
Tissue Kallikrein
Database Links Reactome: R-HSA-381426
Alternative Antibody Names Anti-Kallikrein-1 antibody
Anti-Kidney/Pancreas/Salivary Gland Kallikrein antibody
Anti-Tissue Kallikrein antibody
Anti-KLK1 antibody

Information sourced from Uniprot.org

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