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Anti-KIF5A antibody (933-1032) (STJ24310)

Immunofluorescence analysis of SH-SY5Y cells using KIF5A Rabbit polyclonal antibody (STJ24310) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using KIF5A Rabbit polyclonal antibody (STJ24310) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Western blot analysis of various lysates using KIF5A Rabbit polyclonal antibody (STJ24310) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-KIF5A antibody (933-1032) (STJ24310)

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STJ24310

£112.50 - £522.50

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Rabbit
Applications:	WB/IF
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-KIF5A (933-1032) is suitable for use in Western Blot and Immunofluorescence research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:1000-1:3000 IF/ICC 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	KIF5A
Gene ID:	3798
Uniprot ID:	KIF5A_HUMAN
Immunogen Region:	933-1032
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 933-1032 of human KIF5A (NP_004975.2).
Immunogen Sequence:	TNPYGTRSPECISYTNSLFQ NYQNLYLQATPSSTSDMYFA NSCTSSGATSSGGPLASYQK ANMDNGNATDINDNRSDLPC GYEAEDQAKLFPLHQETAAS

Tissue Specificity	Distributed throughout the CNS but is highly enriched in subsets of neurons.
Function	Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL). Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner. The ZFYVE27-KIF5A complex contributes to the vesicular transport of VAPA, VAPB, SURF4, RAB11A, RAB11B and RTN3 proteins in neurons. Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation.
Protein Name	Kinesin Heavy Chain Isoform 5a Kinesin Heavy Chain Neuron-Specific 1 Neuronal Kinesin Heavy Chain Nkhc
Database Links	Reactome: R-HSA-2132295 Reactome: R-HSA-264876 Reactome: R-HSA-5625970 Reactome: R-HSA-6811434 Reactome: R-HSA-983189
Cellular Localisation	Cytoplasm Perinuclear Region Cytoskeleton Perikaryon Concentrated In The Cell Body Of The Neurons Particularly In The Perinuclear Region
Alternative Antibody Names	Anti-Kinesin Heavy Chain Isoform 5a antibody Anti-Kinesin Heavy Chain Neuron-Specific 1 antibody Anti-Neuronal Kinesin Heavy Chain antibody Anti-Nkhc antibody Anti-KIF5A antibody Anti-NKHC1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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