• STJ70193 (2µg/ml) staining of nuclear NIH3T3 (A) and (2ug/ml) negative control Human Ovary (B) lysate. (35µg protein in RIPA buffer) Detected by chemiluminescence.
  • STJ70193 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml) , showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
  • STJ70193 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-KIF4A antibody (C-Term) (STJ70193)

SKU:
STJ70193-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC
Reactivity: Human/Mouse/Rat/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-KIF4A (C-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-Recommended concentration:1-2µg/ml
IF-Strong expression of the protein seen in the nuclei of U2OS cells. 10µg/ml
FC-Flow cytometric analysis of HEK293 cells. 10ug/ml
ELISA-antibody detection limit dilution 1:64000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Immunogen Region: C-Term
Accession Number: NP_036442.3
Immunogen Sequence: SFFSGCSPIEEEAH
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance