• Immunofluorescence analysis of HeLa cells using KIF4A antibody (STJ112231) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using KIF4A antibody (STJ112231) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human stomach using KIF4A antibody (STJ112231) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat heart using KIF4A antibody (STJ112231) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using KIF4A antibody (STJ112231) at 1:7000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-KIF4A antibody (870-1080) (STJ112231)

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STJ112231

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KIF4A (870-1080) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:3000
IHC-P 1:50-1:200
IF/ICC 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KIF4A
Gene ID: 24137
Uniprot ID: KIF4A_HUMAN
Immunogen Region: 870-1080
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 870-1080 of human KIF4A (NP_036442.3).
Immunogen Sequence: LIGELVSSKIQVSKLESSLK QSKTSCADMQKMLFEERNHF AEIETELQAELVRMEQQHQE KVLYLLSQLQQSQMAEKQLE ESVSEKEQQLLSTLKCQDEE LEKMREVCEQNQQLLRENEI IKQKLTLLQVASRQKHLPKD TLLSPDSSFEYVPPKPKPSR VKEKFLEQSMDIEDLKYCSE HSVNEHEDGDGDDDEGDDEE WKPTKLVKVSR
Tissue Specificity Highly expressed in hematopoietic tissues, fetal liver, spleen, thymus and adult thymus and bone marrow. Lower levels are found in heart, testis, kidney, colon and lung.
Function Iron-sulfur (Fe-S) cluster binding motor protein that has a role in chromosome segregation during mitosis. Translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization.
Protein Name Chromosome-Associated Kinesin Kif4a
Chromokinesin-A
Database Links Reactome: R-HSA-2132295
Reactome: R-HSA-437239
Reactome: R-HSA-6811434
Reactome: R-HSA-983189
Cellular Localisation Nucleus Matrix
Cytoplasm
Cytoskeleton
Spindle
Midbody
Chromosome
Associates With Chromosomes At All Stage Of Mitosis
Chromatin Localization Is Dependent On Iron-Sulfur Cluster Binding
In Anaphase
Associates With The Mitotic Spindle Midzone
In Telophase And Cytokinesis
Co-Localizes With Ciao2b At The Spindle Midzone And Midbody
Co-Localizes With Prc1 In Early Mitosis And At The Spindle Midzone From Anaphase B To Telophase
Does Not Localize To The Nucleolus
Alternative Antibody Names Anti-Chromosome-Associated Kinesin Kif4a antibody
Anti-Chromokinesin-A antibody
Anti-KIF4A antibody
Anti-KIF4 antibody

Information sourced from Uniprot.org

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