• Immunofluorescence analysis of L929 cells using KIF13A Rabbit polyclonal antibody (STJ118325) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using KIF13A Rabbit polyclonal antibody (STJ118325) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from LO2 cells, using KIF13A Rabbit polyclonal antibody (STJ118325) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-KIF13A antibody (1300-1600) (STJ118325)

SKU:
STJ118325

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KIF13A (1300-1600) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KIF13A
Gene ID: 63971
Uniprot ID: KI13A_HUMAN
Immunogen Region: 1300-1600
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1300-1600 of human KIF13A (NP_001099036.1).
Immunogen Sequence: SCGVTYEIVSNIPKATEEIE DRETLALLAARSENEGTSDG ETYIEKYTRGVLQVENILSL ERLRQAVTVKEALSTKARHI RRSLSTPNVHNVSSSRPDLS GFDEDDKGWPENQLDMSDYS SSYQDVACYGTLPRDSPRRN KEGCTSETPHALTVSPFKAF SPQPPKFFKPLMPVKEEHKK RIALEARPLLSQEDSEEEEN ELEAINRKLISSQPYVPVEF ADFSVYNASLENREWFSSK
Tissue Specificity Widely expressed, with highest levels in heart, brain and skeletal muscle.
Function Plus end-directed microtubule-dependent motor protein involved in intracellular transport and regulating various processes such as mannose-6-phosphate receptor (M6PR) transport to the plasma membrane, endosomal sorting during melanosome biogenesis and cytokinesis. Mediates the transport of M6PR-containing vesicles from trans-Golgi network to the plasma membrane via direct interaction with the AP-1 complex. During melanosome maturation, required for delivering melanogenic enzymes from recycling endosomes to nascent melanosomes by creating peripheral recycling endosomal subdomains in melanocytes. Also required for the abcission step in cytokinesis: mediates translocation of ZFYVE26, and possibly TTC19, to the midbody during cytokinesis.
Protein Name Kinesin-Like Protein Kif13a
Kinesin-Like Protein Rbkin
Database Links Reactome: R-HSA-390471
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Midbody
Endosome Membrane
Golgi Apparatus Membrane
Recruited To The Midbody During Cytokinesis
Alternative Antibody Names Anti-Kinesin-Like Protein Kif13a antibody
Anti-Kinesin-Like Protein Rbkin antibody
Anti-KIF13A antibody
Anti-RBKIN antibody

Information sourced from Uniprot.org

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