Host: |
Mouse |
Applications: |
FC/WB/IF/IHC |
Reactivity: |
Cat/Dog/Human/Pig |
Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: |
Mouse monoclonal antibody anti-Keratin 18 is suitable for use in Flow Cytometry, Western Blot, Immunofluorescence and Immunohistochemistry research applications. |
Clonality: |
Monoclonal |
Clone ID: |
EBS-IF-006 |
Conjugation: |
Unconjugated |
Isotype: |
IgG1k |
Formulation: |
PBS with 0.02% Sodium Azide. |
Purification: |
Affinity purified from tissue culture. |
Concentration: |
Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
Dilution Range: |
Flow Cytometry (1-2 µg/million cells in 0.1 ml, fix cells in 4% PFA for 10 min, at 4°C, permeabilize with 0, 2% saponin or digitonin for 15 min, at 4°C). Immunoblotting (1-2 µg/ml). Immunofluorescence (1-2 µg/ml). Immunohistology (formalin-fixed: 2-4 |
Storage Instruction: |
Store for up to 1 year at 2-8°C upon receipt. |
Gene Symbol: |
KRT18 |
Gene ID: |
3875 |
Uniprot ID: |
K1C18_HUMAN |
Immunogen: |
A hybrid mouse (BALB/c x B6) was immunized with a cytokeratin-enriched extract from A431 cells (human vulvar squamous carcinoma). Fusion partner: Sp2/0. |
Post Translational Modifications | Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6. Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7. O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation. |
Function | Involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. |
Protein Name | Keratin - Type I Cytoskeletal 18Cell Proliferation-Inducing Gene 46 ProteinCytokeratin-18Ck-18Keratin-18K18 |
Database Links | Reactome: R-HSA-6805567Reactome: R-HSA-6809371 |
Cellular Localisation | Nucleus MatrixCytoplasmPerinuclear RegionNucleusNucleolus |
Alternative Antibody Names | Anti-Keratin - Type I Cytoskeletal 18 antibodyAnti-Cell Proliferation-Inducing Gene 46 Protein antibodyAnti-Cytokeratin-18 antibodyAnti-Ck-18 antibodyAnti-Keratin-18 antibodyAnti-K18 antibodyAnti-KRT18 antibodyAnti-CYK18 antibodyAnti-PIG46 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance