| Host: | Mouse |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Dog/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-Kelch-like ECH-associated protein 1 (400-500) is suitable for use in Western Blot and ELISA research applications. |
| Clonality : | Monoclonal |
| Clone ID : | PTR2556 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1k |
| Formulation: | Liquid in PBS pH7.4 containing 50% Glycerol and 0.03% Proclin 300. |
| Purification: | Purified using Protein G purification. |
| Dilution Range: | WB 1:500-2000 ELISA 1:5000-20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | KEAP1 |
| Gene ID: | 9817 |
| Uniprot ID: | KEAP1_HUMAN |
| Immunogen Region: | 400-500 |
| Specificity: | This antibody detects endogenous levels of Keap1 at Human, Mouse, Rat |
| Immunogen: | Synthesized peptide derived from human Keap1 AA range: 400-500 |
| Tissue Specificity | Broadly expressed, with highest levels in skeletal muscle. |
| Post Translational Modifications | Non-enzymatic covalent modifications of reactive cysteines by electrophile metabolites inactivate the BCR(KEAP1) complex. Accumulation of fumarate promotes the formation of cysteine S-succination (S-(2-succinyl)cysteine), leading to inactivate the BCR(KEAP1) complex and promote NFE2L2/NRF2 nuclear accumulation and activation. Nitric oxide-dependent 8-Nitro-cGMP formation promotes cysteine guanylation (S-cGMP-cysteine), leading to NFE2L2/NRF2 nuclear accumulation and activation. Itaconate, an anti-inflammatory metabolite generated in response to lipopolysaccharide, alkylates cysteines, activating NFE2L2/NRF2. Methylglyoxal, a reactive metabolite that accumulates when the glycolytic enzyme PGK1 is inhibited, promotes formation of a methylimidazole cross-link between proximal Cys-151 and Arg-135 on another KEAP1 molecule, resulting in an inactive dimer that inactivates the BCR(KEAP1) complex. Degraded via a proteasomal-independent process during selective autophagy: interaction with phosphorylated SQSTM1/p62 sequesters KEAP1 in inclusion bodies, leading to its degradation. Auto-ubiquitinated by the BCR(KEAP1) complex. Quinone-induced oxidative stress, but not sulforaphane, increases its ubiquitination. Ubiquitination and subsequent degradation is most pronounced following prolonged exposure of cells to oxidative stress, particularly in glutathione-deficient cells that are highly susceptible to oxidative stress. Deubiquitinated by USP25.leading to stabilization. |
| Function | Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that regulates the response to oxidative stress by targeting NFE2L2/NRF2 for ubiquitination. KEAP1 acts as a key sensor of oxidative and electrophilic stress: in normal conditions, the BCR(KEAP1) complex mediates ubiquitination and degradation of NFE2L2/NRF2, a transcription factor regulating expression of many cytoprotective genes. In response to oxidative stress, different electrophile metabolites trigger non-enzymatic covalent modifications of highly reactive cysteine residues in KEAP1, leading to inactivate the ubiquitin ligase activity of the BCR(KEAP1) complex, promoting NFE2L2/NRF2 nuclear accumulation and expression of phase II detoxifying enzymes. In response to selective autophagy, KEAP1 is sequestered in inclusion bodies following its interaction with SQSTM1/p62, leading to inactivation of the BCR(KEAP1) complex and activation of NFE2L2/NRF2. The BCR(KEAP1) complex also mediates ubiquitination of SQSTM1/p62, increasing SQSTM1/p62 sequestering activity and degradation. The BCR(KEAP1) complex also targets BPTF and PGAM5 for ubiquitination and degradation by the proteasome. |
| Protein Name | Kelch-Like Ech-Associated Protein 1 Cytosolic Inhibitor Of Nrf2 Inrf2 Kelch-Like Protein 19 |
| Database Links | Reactome: R-HSA-5689880 Reactome: R-HSA-8951664 Reactome: R-HSA-9679191 Reactome: R-HSA-9755511 Reactome: R-HSA-9759194 Reactome: R-HSA-983168 |
| Cellular Localisation | Cytoplasm Nucleus Mainly Cytoplasmic In Response To Selective Autophagy Relocalizes To Inclusion Bodies Following Interaction With Sqstm1/P62 |
| Alternative Antibody Names | Anti-Kelch-Like Ech-Associated Protein 1 antibody Anti-Cytosolic Inhibitor Of Nrf2 antibody Anti-Inrf2 antibody Anti-Kelch-Like Protein 19 antibody Anti-KEAP1 antibody Anti-INRF2 antibody Anti-KIAA0132 antibody Anti-KLHL19 antibody |
Information sourced from Uniprot.org
