• Immunofluorescence analysis of U2OS cells using KDM6B antibody (STJ114636) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using KDM6B antibody (STJ114636) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of mouse brain, using KDM6B antibody (STJ114636) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of various cell lines, using Kdm6b antibody (STJ114636) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-Kdm6b antibody (90-1641) (STJ114636)

SKU:
STJ114636

£112.50 - £522.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Kdm6b (90-1641) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Kdm6b
Gene ID: 216850
Uniprot ID: KDM6B_MOUSE
Immunogen Region: 90-1641
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 90-1641 of mouse Kdm6b (NP_001017426.1).
Immunogen Sequence: LESLHGCVQALLREPAQPGL WEQLGQLYESEHDSEEAVCC YHRALRYGGSFAELGPRIGR LQQAQLWNFHAGSCQHRAKV LPPLEQVWNLLHLEHKRNYG A
Function Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. Plays a demethylase-independent role in chromatin remodeling to regulate T-box family member-dependent gene expression by acting as a link between T-box factors and the SMARCA4-containing SWI/SNF remodeling complex.
Protein Name Lysine-Specific Demethylase 6b
Jmjc Domain-Containing Protein 3
Jumonji Domain-Containing Protein 3
Histone H3-Trimethyl-L-Lysine(27 Demethylase 6b
Database Links Reactome: R-MMU-2559580
Reactome: -MMU-3214842
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Lysine-Specific Demethylase 6b antibody
Anti-Jmjc Domain-Containing Protein 3 antibody
Anti-Jumonji Domain-Containing Protein 3 antibody
Anti-Histone H3-Trimethyl-L-Lysine(27 Demethylase 6b antibody
Anti-Kdm6b antibody
Anti-Jmjd3 antibody
Anti-Kiaa0346 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance