• Immunofluorescence analysis of U2OS cells using KDM3A Rabbit polyclonal antibody (STJ113876) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using KDM3A Rabbit polyclonal antibody (STJ113876) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using KDM3A Rabbit polyclonal antibody (STJ113876) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-KDM3A antibody (975-1283) (STJ113876)

SKU:
STJ113876

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KDM3A (975-1283) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KDM3A
Gene ID: 55818
Uniprot ID: KDM3A_HUMAN
Immunogen Region: 975-1283
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 975-1283 of human KDM3A (NP_060903.2).
Immunogen Sequence: PVMVSGVHHKLNSELWKPES FRKEFGEQEVDLVNCRTNEI ITGATVGDFWDGFEDVPNRL KNEKEPMVLKLKDWPPGEDF RDMMPSRFDDLMANIPLPEY TRRDGKLNLASRLPNYFVRP DLGPKMYNAYGLITPEDRKY GTTNLHLDVSDAANVMVYVG IPKGQCEQEEEVLKTIQDGD SDELTIKRFIEGKEKPGALW HIYAAKDTEKIREFLKKVSE EQGQENPADHDPIHDQSWY
Function Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TNP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.
Protein Name Lysine-Specific Demethylase 3a
Jmjc Domain-Containing Histone Demethylation Protein 2a
Jumonji Domain-Containing Protein 1a
Histone H3-Dimethyl-L-Lysine(9 Demethylase 3a
Database Links Reactome: R-HSA-3214842
Reactome: R-HSA-9029569
Cellular Localisation Cytoplasm
Nucleus
Nuclear In Round Spermatids
When Spermatids Start To Elongate
Localizes To The Cytoplasm Where It Forms Distinct Foci Which Disappear In Mature Spermatozoa
Alternative Antibody Names Anti-Lysine-Specific Demethylase 3a antibody
Anti-Jmjc Domain-Containing Histone Demethylation Protein 2a antibody
Anti-Jumonji Domain-Containing Protein 1a antibody
Anti-Histone H3-Dimethyl-L-Lysine(9 Demethylase 3a antibody
Anti-KDM3A antibody
Anti-JHDM2A antibody
Anti-JMJD1 antibody
Anti-JMJD1A antibody
Anti-KIAA0742 antibody
Anti-TSGA antibody

Information sourced from Uniprot.org

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