| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
KCNK6 |
| Gene ID: |
9424 |
| Uniprot ID: |
KCNK6_HUMAN |
| Immunogen Region: |
254-313 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 254-313 of human KCNK6 (NP_004814.1). |
| Immunogen Sequence: |
QTFRHVSDLHGLTELILLPP PCPASFNADEDDRVDILGPQ PESHQQLSASSHTDYASIPR |
| Tissue Specificity | Widespread expression, detected in all tissues tested except for skeletal muscle. Strongest expression in placenta, pancreas, heart, colon and spleen, lower levels detected in peripheral blood leukocytes, lung, liver, kidney and thymus. Lowest expression detected in brain. |
| Post Translational Modifications | N-glycosylation is necessary for targeting to lysosomes. |
| Function | K(+) channel that conducts outward rectifying currents at the membranes of the endolysosomal system. Active in lysosomes where it regulates lysosome numbers and size. In macrophages, enables K(+) efflux coupled to ATP-induced NLRP3 inflammasome activation upon bacterial infection. Cooperates with ATP-gated P2RX7 channels to activate NLRP3 inflammasome, with P2RX7 conducting Ca(2+) and Na(+) influx that sets the membrane potential for K(+) efflux. Isoform 2: Does not display channel activity. |
| Protein Name | Potassium Channel Subfamily K Member 6Inward Rectifying Potassium Channel Protein Twik-2Twik-Originated Similarity Sequence |
| Database Links | Reactome: R-HSA-1299308Reactome: R-HSA-5576886 |
| Cellular Localisation | Late Endosome MembraneMulti-Pass Membrane ProteinLysosome Membrane |
| Alternative Antibody Names | Anti-Potassium Channel Subfamily K Member 6 antibodyAnti-Inward Rectifying Potassium Channel Protein Twik-2 antibodyAnti-Twik-Originated Similarity Sequence antibodyAnti-KCNK6 antibodyAnti-TOSS antibodyAnti-TWIK2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and PSME3 knockout (KO) 293T cells, using [KO Validated] PSME3 Rabbit polyclonal antibody (STJ119994) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/98779/382828/STJ119994_1__38128.1713151329.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and PSME3 knockout (KO) 293T cells, using [KO Validated] PSME3 Rabbit polyclonal antibody (STJ119994) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
