• Western blot analysis of extracts of U-87MG cells, using KAZN antibody (STJ118468) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-KAZN antibody (586-775) (STJ118468)

SKU:
STJ118468

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KAZN (586-775) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KAZN
Gene ID: 23254
Uniprot ID: KAZRN_HUMAN
Immunogen Region: 586-775
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 586-775 of human KAZN (NP_963922.2).
Immunogen Sequence: YQVNFSREALQERRARCETQ NIDPVVWTNQRVLKWVRDID LKEYADNLTNSGVHGAVLVL EPTFNAEAMATALGIPSGKH ILRRHLAEEMSAVFHPANST GIREAERFGTPPGRASSVTR AGKEENSSGLKYKAGRLPLG KIGRGFSSKDPDFHDDYGSL QNEDCGDDDPQSRLEQCRLE GYNSLEVTNV
Tissue Specificity Isoform 2, isoform 3 and isoform 4 are expressed in several cell lines including keratinocytes and bladder and epidermoid carcinoma (at protein level). Isoform 2, isoform 3 and isoform 4 are expressed in hair follicle and interfollicular epidermis (at protein level).
Function Component of the cornified envelope of keratinocytes. May be involved in the interplay between adherens junctions and desmosomes. The function in the nucleus is not known.
Protein Name Kazrin
Database Links Reactome: R-HSA-6809371
Cellular Localisation Cytoplasm
Cytoskeleton
Isoform 2: Cytoplasm
Cell Junction
Desmosome
Nucleus
Observed At The Apical Plasma Membrane Of Keratinocytes
Partially Colocalizes With Ppl And Dp At Desmosomes
And With Pp At The Interdesmosomal Plasma Membrane
Colocalizes With Cortical Actin-Based Membrane Structures
Isoform 3: Cytoplasm
Isoform 4: Cytoplasm
Alternative Antibody Names Anti-Kazrin antibody
Anti-KAZN antibody
Anti-C1orf196 antibody
Anti-KAZ antibody
Anti-KIAA1026 antibody
Anti-HRIHFB2003 antibody

Information sourced from Uniprot.org

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