• Western blot analysis of extracts of various cell lines, using Integrin alpha 5 (ITGA5/CD49e) antibody (STJ11104662) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-ITGA5 antibody (42-995) (STJ11104662)

SKU:
STJ11104662

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Integrin Alpha-5 (42-995) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ITGA5
Gene ID: 3678
Uniprot ID: ITA5_HUMAN
Immunogen Region: 42-995
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 42-995 of human Integrin alpha 5 (ITGA5/CD49e) (NP_002196.4).
Immunogen Sequence: FNLDAEAPAVLSGPPGSFFG FSVEFYRPGTDGVSVLVGAP KANTSQPGVLQGGAVYLCPW GASPTQCTPIEFDSKGSRLL ESSLSSSEGEEPVEYKSLQW FGATVRAHGSSILACAPLYS WRTEKEPLSDPVGTCYLSTD NFTRILEYAPCRSDFSWAAG QGYCQGGFSAEFTKTGRVVL GGPGSYFWQGQILSATQEQI AESYYPEYLINLVQGQLQTR QASSIYDDSYLGYSVAVGE
Tissue Specificity Expressed in placenta (at protein level).
Post Translational Modifications Proteolytic cleavage by PCSK5 mediates activation of the precursor.
Function Integrin alpha-5/beta-1 (ITGA5:ITGB1) is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. ITGA5:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1. ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1. ITGA5:ITGB1 acts as a receptor for fibronectin (FN1) and mediates R-G-D-dependent cell adhesion to FN1. ITGA5:ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling. ITGA5:ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling. (Microbial infection) Integrin ITGA5:ITGB1 acts as a receptor for Human metapneumovirus. (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human parvovirus B19. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Protein Name Integrin Alpha-5
Cd49 Antigen-Like Family Member E
Fibronectin Receptor Subunit Alpha
Integrin Alpha-F
Vla-5
Cd Antigen Cd49e Cleaved Into - Integrin Alpha-5 Heavy Chain - Integrin Alpha-5 Light Chain
Database Links Reactome: R-HSA-1566948
Reactome: R-HSA-1566977
Reactome: R-HSA-202733
Reactome: R-HSA-216083
Reactome: R-HSA-445144
Reactome: R-HSA-8941332
Reactome: R-HSA-9634597
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Cell Junction
Focal Adhesion
Alternative Antibody Names Anti-Integrin Alpha-5 antibody
Anti-Cd49 Antigen-Like Family Member E antibody
Anti-Fibronectin Receptor Subunit Alpha antibody
Anti-Integrin Alpha-F antibody
Anti-Vla-5 antibody
Anti-Cd Antigen Cd49e Cleaved Into - Integrin Alpha-5 Heavy Chain - Integrin Alpha-5 Light Chain antibody
Anti-ITGA5 antibody
Anti-FNRA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance