• Immunofluorescence analysis of L929 cells using IPO8 Rabbit polyclonal antibody (STJ116881) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using IPO8 Rabbit polyclonal antibody (STJ116881) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using IPO8 Rabbit polyclonal antibody (STJ116881) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using IPO8 Rabbit polyclonal antibody (STJ116881) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-IPO8 antibody (878-1037) (STJ116881)

SKU:
STJ116881

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IPO8 (878-1037) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IPO8
Gene ID: 10526
Uniprot ID: IPO8_HUMAN
Immunogen Region: 878-1037
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 878-1037 of human IPO8 (NP_006381.2).
Immunogen Sequence: TRQLVNREDRSKAEKADMEE NEEISSDEEETNVTAQAMQS NNGRGEDEEEEDDDWDEEVL EETALEGFSTPLDLDNSVDE YQFFTQALITVQSRDAAWYQ LLMAPLSEDQRTALQEVYTL AEHRRTVAEAKKKIEQQGGF TFENKGVLSAFNFGTVPSNN
Function Involved in nuclear protein import, either by acting as autonomous nuclear transport receptor or as an adapter-like protein in association with the importin-beta subunit KPNB1. Acting autonomously, may serve as receptor for nuclear localization signals (NLS) and promote translocation of import substrates through the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro mediates the nuclear import of the signal recognition particle protein SRP19. May also be involved in cytoplasm-to-nucleus shuttling of a broad spectrum of other cargos, including Argonaute-microRNAs complexes, the JUN protein, RELA/NF-kappa-B p65 subunit, the translation initiation factor EIF4E and a set of receptor-activated mothers against decapentaplegic homolog (SMAD) transcription factors that play a critical role downstream of the large family of transforming growth factor beta and bone morphogenetic protein (BMP) cytokines (Probable).
Protein Name Importin-8
Imp8
Ran-Binding Protein 8
Ranbp8
Database Links Reactome: R-HSA-5578749
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Importin-8 antibody
Anti-Imp8 antibody
Anti-Ran-Binding Protein 8 antibody
Anti-Ranbp8 antibody
Anti-IPO8 antibody
Anti-RANBP8 antibody

Information sourced from Uniprot.org

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