| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ILKAP (1-392) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ILKAP |
| Gene ID: |
80895 |
| Uniprot ID: |
ILKAP_HUMAN |
| Immunogen Region: |
1-392 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-392 of human ILKAP (NP_110395.1). |
| Immunogen Sequence: |
MDLFGDLPEPERSPRPAAGK EAQKGPLLFDDLPPASSTDS GSGGPLLFDDLPPASSGDSG SLATSISQMVKTEGKGAKRK TSEEEKNGSEELVEKKVCKA SSVIFGLKGYVAERKGEREE MQDAHVILNDITEECRPPSS LITRVSYFAVFDGHGGIRAS KFAAQNLHQNLIRKFPKGDV ISVEKTVKRCLLDTFKHTDE EFLKQASSQKPAWKDGSTAT CVLAVDNILYIANLGDSRA |
| Tissue Specificity | Widely expressed. Highest levels expressed in striated muscle. Much lower levels evident in various smooth muscle tissues. |
| Function | Protein phosphatase that may play a role in regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. Selectively associates with integrin linked kinase (ILK), to modulate cell adhesion and growth factor signaling. Inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation. |
| Protein Name | Integrin-Linked Kinase-Associated Serine/Threonine Phosphatase 2cIlkap |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Integrin-Linked Kinase-Associated Serine/Threonine Phosphatase 2c antibodyAnti-Ilkap antibodyAnti-ILKAP antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and SEPHS1 knockout (KO) 293T cells, using [KO Validated] SEPHS1 Rabbit polyclonal antibody (STJ11100795) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89731/358209/STJ11100795_1__53467.1713122532.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and SEPHS1 knockout (KO) 293T cells, using [KO Validated] SEPHS1 Rabbit polyclonal antibody (STJ11100795) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
