| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Monoclonal |
| Clone ID: |
S6MR |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ILK |
| Gene ID: |
3611 |
| Uniprot ID: |
ILK_HUMAN |
| Immunogen Region: |
350-452 |
| Specificity: |
A synthetic peptide corresponding to a sequence within amino acids 350-452 of human Integrin linked ILK (Q13418). |
| Immunogen Sequence: |
MYAPAWVAPEALQKKPEDTN RRSADMWSFAVLLWELVTRE VPFADLSNMEIGMKVALEGL RPTIPPGISPHVCKLMKICM NEDPAKRPKFDMIVPILEKM QDK |
| Tissue Specificity | Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver. |
| Post Translational Modifications | Phosphorylation by PAK1 modulates ILK subcellular location by promoting its nuclear export. |
| Function | Scaffold protein which mediates protein-protein interactions during a range of cellular events including focal adhesion assembly, cell adhesion and cell migration. Regulates integrin-mediated signal transduction by contributing to inside-out integrin activation. Recruits PARVA and LIMS1/PITCH to form the heterotrimeric IPP (ILK-PINCH-PARVIN) complex which binds to F-actin via the C-terminal tail of LIMS1 and the N-terminal region of PARVA, promoting F-actin filament bundling, a process required to generate force for actin cytoskeleton reorganization and subsequent dynamic cell adhesion events such as cell spreading and migration. Binding to PARVA promotes effective assembly of ILK into focal adhesions while PARVA-bound ILK can simultaneously engage integrin-beta cytoplasmic tails to mediate cell adhesion. Plays a role with PARVG in promoting the cell adhesion and spreading of leukocytes. Acts as an upstream effector of both AKT1/PKB and GSK3. Mediates trafficking of caveolae to the cell surface in an ITGB1-dependent manner by promoting the recruitment of IQGAP1 to the cell cortex which cooperates with its effector DIAPH1 to locally stabilize microtubules and allow stable insertion of caveolae into the plasma membrane. Required for the maintenance of mitotic spindle integrity by promoting phosphorylation of TACC3 by AURKA. Associates with chromatin and may act as a negative regulator of transcription when located in the nucleus. |
| Protein Name | Scaffold Protein IlkIlk-1Ilk-2Inactive Integrin-Linked KinaseP59ilk |
| Database Links | Reactome: R-HSA-446343Reactome: R-HSA-446353 |
| Cellular Localisation | Cell JunctionFocal AdhesionCell MembranePeripheral Membrane ProteinCytoplasmic SideCell ProjectionLamellipodiumCytoplasmMyofibrilSarcomereNucleusCytoskeletonMicrotubule Organizing CenterCentrosomeCell Cortex |
| Alternative Antibody Names | Anti-Scaffold Protein Ilk antibodyAnti-Ilk-1 antibodyAnti-Ilk-2 antibodyAnti-Inactive Integrin-Linked Kinase antibodyAnti-P59ilk antibodyAnti-ILK antibodyAnti-ILK1 antibodyAnti-ILK2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
