| Host: | Mouse |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-Interleukin-1 beta (150-250) is suitable for use in Western Blot and ELISA research applications. |
| Clonality : | Monoclonal |
| Clone ID : | PTR2541 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1k |
| Formulation: | Liquid in PBS pH7.4 containing 50% Glycerol and 0.03% Proclin 300. |
| Purification: | Purified using Protein G purification. |
| Dilution Range: | WB 1:500-2000 ELISA 1:5000-20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | IL1B |
| Gene ID: | 3553 |
| Uniprot ID: | IL1B_HUMAN |
| Immunogen Region: | 150-250 |
| Specificity: | This antibody detects endogenous levels of IL-1 Beta at Human, Mouse, Rat |
| Immunogen: | Synthesized peptide derived from human IL-1 Beta AA range: 150-250 |
| Tissue Specificity | Expressed in activated monocytes/macrophages (at protein level). |
| Post Translational Modifications | Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated. (Microbial infection) Cleavage by S.pyogenes cysteine protease SpeB promotes its activation independently of CASP1. |
| Function | Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. Acts as a sensor of S.pyogenes infection in skin: cleaved and activated by pyogenes SpeB protease, leading to an inflammatory response that prevents bacterial growth during invasive skin infection. |
| Protein Name | Interleukin-1 Beta Il-1 Beta Catabolin |
| Database Links | Reactome: R-HSA-448706 Reactome: R-HSA-5620971 Reactome: R-HSA-5660668 Reactome: R-HSA-6783783 Reactome: R-HSA-6785807 Reactome: R-HSA-9020702 Reactome: R-HSA-9660826 |
| Cellular Localisation | Cytoplasm Cytosol Secreted Lysosome Extracellular Exosome The Precursor Is Cytosolic In Response To Inflammasome-Activating Signals Such As Atp For Nlrp3 Inflammasome Or Bacterial Flagellin For Nlrc4 Inflammasome Cleaved And Secreted Mature Form Is Secreted And Released In The Extracellular Milieu By Passing Through The Gasdermin-D (Gsdmd) Pore In Contrast The Precursor Form Is Not Released Due To The Presence Of An Acidic Region That Is Proteolytically Removed By Casp1 During Maturation The Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10 |
| Alternative Antibody Names | Anti-Interleukin-1 Beta antibody Anti-Il-1 Beta antibody Anti-Catabolin antibody Anti-IL1B antibody Anti-IL1F2 antibody |
Information sourced from Uniprot.org
