• STJ72126 (1ug/ml) staining of Mouse Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ72126 (2µg/ml) staining of Jurkat nuclear lysate (A) + Peptide (B) , and (0. 1ug/ml) staining of K562 nuclear lysate (C) + Peptide (D) , (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ72126 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ72126 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ72126 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-IKZF4 antibody (Internal) (STJ72126)

SKU:
STJ72126-100

£312.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC
Reactivity: Human/Mouse/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-IKZF4 (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:2000.
WB: Approx 60kDa band observed in nuclear lysates of cell line Jurkat, approx.. 60+70kDa in nuclear lysates of cell line K562, and approx. 55kDa in Mouse Heart lysates (calculated MW of 64.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: IKZF4
Gene ID: 64375
Uniprot ID: IKZF4_HUMAN
Immunogen Region: Internal
Accession Number: NP_071910.3; NP_001338019.1; NP_001338020.1; NP_001338021.1
Specificity: This antibody is expected to recognise reported isoforms NP_071910.3; NP_001338019.1; NP_001338020.1; NP_001338021.1
Immunogen Sequence: RPTFIDRLANSLTKR
Function DNA-binding protein that binds to the 5'GGGAATRCC-3' Ikaros-binding sequence. Transcriptional repressor. Interacts with SPI1 and MITF to repress transcription of the CTSK and ACP5 promoters via recruitment of corepressors SIN3A and CTBP2. May be involved in the development of central and peripheral nervous systems. Essential for the inhibitory function of regulatory T-cells (Treg). Mediates FOXP3-mediated gene silencing in regulatory T-cells (Treg) via recruitment of corepressor CTBP1.
Protein Name Zinc Finger Protein Eos
Ikaros Family Zinc Finger Protein 4
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Zinc Finger Protein Eos antibody
Anti-Ikaros Family Zinc Finger Protein 4 antibody
Anti-IKZF4 antibody
Anti-KIAA1782 antibody
Anti-ZNFN1A4 antibody

Information sourced from Uniprot.org

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