| Host: | Goat |
| Applications: | Pep-ELISA/WB/IF/FC |
| Reactivity: | Human/Mouse/Dog/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-IKZF4 (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx 60kDa band observed in nuclear lysates of cell line Jurkat, approx.. 60+70kDa in nuclear lysates of cell line K562, and approx. 55kDa in Mouse Heart lysates (calculated MW of 64. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | IKZF4 |
| Gene ID: | 64375 |
| Uniprot ID: | IKZF4_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_071910.3; NP_001338019.1; NP_001338020.1; NP_001338021.1 |
| Specificity: | This antibody is expected to recognise reported isoforms NP_071910.3; NP_001338019.1; NP_001338020.1; NP_001338021.1 |
| Immunogen Sequence: | RPTFIDRLANSLTKR |
| Function | DNA-binding protein that binds to the 5'GGGAATRCC-3' Ikaros-binding sequence. Transcriptional repressor. Interacts with SPI1 and MITF to repress transcription of the CTSK and ACP5 promoters via recruitment of corepressors SIN3A and CTBP2. May be involved in the development of central and peripheral nervous systems. Essential for the inhibitory function of regulatory T-cells (Treg). Mediates FOXP3-mediated gene silencing in regulatory T-cells (Treg) via recruitment of corepressor CTBP1. |
| Protein Name | Zinc Finger Protein EosIkaros Family Zinc Finger Protein 4 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Zinc Finger Protein Eos antibodyAnti-Ikaros Family Zinc Finger Protein 4 antibodyAnti-IKZF4 antibodyAnti-KIAA1782 antibodyAnti-ZNFN1A4 antibody |
Information sourced from Uniprot.org