• Immunofluorescence analysis of NIH/3T3 cells using IKBIP Rabbit polyclonal antibody (STJ11105730) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using IKBIP Rabbit polyclonal antibody (STJ11105730) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-IKBIP antibody (115-350) (STJ11105730)

SKU:
STJ11105730

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Inhibitor Of Nuclear Factor Kappa-B Kinase-Interacting Protein (115-350) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IF/ICC 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IKBIP
Gene ID: 121457
Uniprot ID: IKIP_HUMAN
Immunogen Region: 115-350
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 115-350 of human IKBIP (NP_963906.1).
Immunogen Sequence: IIAHLKRLQEEINEVKTWSN RITEKQDILNNSLTTLSQDI TKVDQSTTSMAKDVGLKITS VKTDIRRISGLVTDVISLTD SVQELENKIEKVEKNTVKNI GDLLSSSIDRTATLRKTASE NSQRINSVKKTLTELKSDFD KHTDRFLSLEGDRAKVLKTV TFANDLKPKVYNLKKDFSRL EPLVNDLTLRIGRLVTDLLQ REKEIAFLSEKISNLTIVQA EIKDIKDEIAHISDMN
Tissue Specificity Expressed in vein endothelial cells. Isoform 4 is expressed in lung, kidney, spleen, thymus and skeletal muscle.
Post Translational Modifications N-glycosylated. Isoform 4 is glycosylated at Asn-154.
Function Target of p53/TP53 with pro-apoptotic function.
Protein Name Inhibitor Of Nuclear Factor Kappa-B Kinase-Interacting Protein
I Kappa-B Kinase-Interacting Protein
Ikbkb-Interacting Protein
Ikk-Interacting Protein
Database Links Reactome: R-HSA-9758274
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Membrane Protein
Isoform 4 Deletion Of The Hydrophobic
Or Transmembrane Region Between Aa 45-63 Results In Uniform Distribution Throughout The Cell
Suggesting That This Region Is Responsible For Endoplasmic Reticulum Localization
Alternative Antibody Names Anti-Inhibitor Of Nuclear Factor Kappa-B Kinase-Interacting Protein antibody
Anti-I Kappa-B Kinase-Interacting Protein antibody
Anti-Ikbkb-Interacting Protein antibody
Anti-Ikk-Interacting Protein antibody
Anti-IKBIP antibody
Anti-IKIP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance