Western blot analysis of various lysates using IFT81 Rabbit polyclonal antibody (STJ116254) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IFT81 antibody (550-676) (STJ116254)

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STJ116254
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IFT81
Gene ID: 28981
Uniprot ID: IFT81_HUMAN
Immunogen Region: 550-676
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 550-676 of human IFT81 (NP_054774.2).
Immunogen Sequence: VRRLREECLQEESRYHYTNC MIKNLEVQLRRATDEMKAYI SSDQQEKRKAIREQYTKNTA EQENLGKKLREKQKVIRESH GPNMKQAKMWRDLEQLMECK KQCFLKQQSQTSIGQVIQEG GEDRLIL
Tissue Specificity Highly expressed in testis, moderately in ovary, heart, liver, skeletal muscle, kidney and pancreas, low in prostate, brain, placenta and lung and not detected in spleen, thymus, small intestine and colon. Isoform CDV-1R is abundantly expressed in testis.
Function Component of the intraflagellar transport (IFT) complex B: together with IFT74, forms a tubulin-binding module that specifically mediates transport of tubulin within the cilium. Binds tubulin via its CH (calponin-homology)-like region. Required for ciliogenesis. Required for proper regulation of SHH signaling. Plays an important role during spermatogenesis by modulating the assembly and elongation of the sperm flagella.
Protein Name Intraflagellar Transport Protein 81 Homolog
Carnitine Deficiency-Associated Protein Expressed In Ventricle 1
Cdv-1
Database Links Reactome: R-HSA-5620924
Cellular Localisation Cell Projection
Cilium
Cytoplasm
Alternative Antibody Names Anti-Intraflagellar Transport Protein 81 Homolog antibody
Anti-Carnitine Deficiency-Associated Protein Expressed In Ventricle 1 antibody
Anti-Cdv-1 antibody
Anti-IFT81 antibody
Anti-CDV1 antibody

Information sourced from Uniprot.org

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