• Western blot analysis of extracts of mouse brain, using IDUA antibody (STJ115724) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-IDUA antibody (354-653) (STJ115724)

SKU:
STJ115724

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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IDUA (354-653) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IDUA
Gene ID: 3425
Uniprot ID: IDUA_HUMAN
Immunogen Region: 354-653
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 354-653 of human IDUA (NP_000194.2).
Immunogen Sequence: SYHPHPFAQRTLTARFQVNN TRPPHVQLLRKPVLTAMGLL ALLDEEQLWAEVSQAGTVLD SNHTVGVLASAHRPQGPADA WRAAVLIYASDDTRAHPNRS VAVTLRLRGVPPGPGLVYVT RYLDNGLCSPDGEWRRLGRP VFPTAEQFRRMRAAEDPVAA APRPLPAGGRLTLRPALRLP SLLLVHVCARPEKPPGQVTR LRALPLTQGQLVLVWSDEHV GSKCLWTYEIQFSQDGKAY
Tissue Specificity Ubiquitous.
Post Translational Modifications N-glycosylation at Asn-372 contributes to substrate binding and is required for full enzymatic activity.
Protein Name Alpha-L-Iduronidase
Database Links Reactome: R-HSA-2024096
Reactome: R-HSA-2024101
Reactome: R-HSA-2206302
Cellular Localisation Lysosome
Alternative Antibody Names Anti-Alpha-L-Iduronidase antibody
Anti-IDUA antibody

Information sourced from Uniprot.org

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